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Advanced Bacterial Genetics

Use of Transposons and Phage for Genomic Engineering: Volume 421

By: Kelly T. Hughes (Volume Editor)

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Hardcover | 1 March 2007 | Edition Number 421

At a Glance

Hardcover
320 Pages

Dimensions(cm)
23.04 x 16.0 x 2.44

Hardcover

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The critically acclaimed laboratory standard for more than fifty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 400 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences. This new volume presents methods related to the use of bacterial genetics for genomic engineering. The book includes sections on strain collections and genetic nomenclature; transposons; and phage.

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You Can Find This Book In

Non-Fiction Science Biology, Life Sciences Life Sciences in General Genetics excluding Medical Biochemistry Enzymology Microbiology excluding Medical Bacteriology excluding Medical Engineering & Technology Biochemical Engineering Biotechnology

Genetic Engineering Medicine Pre-Clinical Medicine & Basic Sciences Medical Genetics

Strain Collections and Genetic Nomenclature
Strain Collections and Genetic Nomenclature
Transposons
Use of Antibiotic Resistant Transposons for Mutagenesis
In vivo Mutagenesis Using EZ-Tn5TM
Identification of essential genes in bacteria
Isolation and use of linked transposons
Localized mutagenesis
Generation of deletions and duplications using transposons as portable regions of homology with emphasis on Mud and Tn10 transposons
Target-Directed Proteolysis in vivo
Sets of transposon generated sequence-tagged mutants for structure-function analysis and engineering
Using Genomic Microarrays to Study Insertional/Transposon Mutant Libraries
Screening Transposon Mutant Libraries Using Full-Genome Oligonucleotide Microarrays
Creating recombination-activated genes and sequence-defined mutant libraries using transposons
Use of transposons to study genetic regulation fusions
Genomic screening for regulatory genes using the T-POP transposon
Phage
Recombineering: in vivo genetic engineering in E. coli, S. enterica and beyond
++-Red Genetic Engineering in Salmonella enterica serovar Typhimurium
Integrase
Challenge phage
MudP22
Phage metagenomics
Table of Contents provided by Publisher. All Rights Reserved.

Advanced Bacterial Genetics

Use of Transposons and Phage for Genomic Engineering: Volume 421

At a Glance

Hardcover

Shipping

How to return your order

Defective items

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