Contributors | p. iii |
Contents of Other Volumes | p. ix |
Roles of High-Performance Liquid Chromatography in Nuclear Medicine | p. 1 |
Introduction | p. 2 |
Characteristics of Radiopharmaceuticals | p. 3 |
Instrumentation | p. 5 |
Preparation and Purification | p. 6 |
Analysis of [superscript 99m]Tc-Labeled Pharmaceuticals | p. 28 |
Conclusion | p. 31 |
Acknowledgments | p. 32 |
References | p. 32 |
Calibration of Separation Systems in Gel Permeation Chromatography for Polymer Characterization | p. 37 |
Introduction | p. 38 |
Calibration Using Standards with Narrow Distributions | p. 39 |
Calibration Using Standards with Broad Distribution | p. 46 |
Universal Calibration | p. 52 |
Special Procedures of Calibration | p. 71 |
Influence of Operational Variables on Results of Calibration | p. 72 |
Conclusions | p. 78 |
Acknowledgments | p. 79 |
Applications of Universal Calibration Principles in Synthetic Polymer Characterization | p. 80 |
References | p. 80 |
Isomer-Specific Assay of 2,4-D Herbicide Products By HPLC: Regulatory Methodology | p. 91 |
Introduction | p. 91 |
Ion-Exchange Chromatography Versus Reverse-Phase Chromatography | p. 93 |
Method Criteria | p. 94 |
Impurities in 2,4-D | p. 94 |
Separation of Impurities | p. 94 |
Alternative Columns | p. 100 |
Column Stability | p. 107 |
Precision and Linearity | p. 107 |
Mobile Phase Modifier | p. 108 |
Conclusions | p. 108 |
References | p. 108 |
Hydrophobic Interaction Chromatography | p. 111 |
Introduction | p. 112 |
Definition of Hydrophobic Interaction and the Structure of Water | p. 112 |
Changes in the Water Structure upon Solubilization of a Nonpolar Compound in Water | p. 113 |
Chemical Nature of the Hydrophobic Interaction | p. 113 |
Charged Amphiphilic Adsorbents | p. 115 |
Neutral Amphiphilic Adsorbents | p. 116 |
Multipoint Interaction | p. 117 |
Establishment of Appropriate Experimental Conditions for a Run | p. 118 |
Applications | p. 119 |
Acknowledgments | p. 121 |
References | p. 121 |
Liquid Chromatography with Programmed Composition of the Mobile Phase | p. 125 |
Introduction | p. 126 |
Retention Characteristics in Isocratic-Elution Chromatography and in Chromatography with Programmed Composition of the Mobile Phase | p. 128 |
Practical Aspects of Chromatography with Programmed Composition of the Mobile Phase | p. 235 |
Conclusions | p. 248 |
Summary | p. 248 |
List of Symbols Used | p. 249 |
References | p. 253 |
Chromatographic Separation of Aldosterone and Its Metabolites | p. 261 |
Introduction | p. 262 |
Metabolism and Mechanism of Action of Aldosterone | p. 263 |
Separation and Column Chromatography of Reduced and Polar Aldosterone Metabolites | p. 264 |
High-Pressure Liquid Chromatography of Reduced and Polar Aldosterone Metabolites | p. 268 |
Improved Separations of Aldosterone Metabolites Using High-Pressure Liquid Chromatography | p. 273 |
High-Pressure Liquid Chromatography of Aldosterone Metabolites Synthesized in Vitro | p. 275 |
Aldosterone Metabolites in Male Rat Urine | p. 281 |
Conclusions | p. 282 |
Acknowledgments | p. 283 |
References | p. 283 |
Author Index | p. 287 |
Subject Index | p. 307 |
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