| Introduction | p. 1 |
| Historic Examples of Ultracentrifugation | p. 2 |
| Investigations on Gold Colloids in 1924 | p. 3 |
| Investigations on the Structure of DNA in 1957 | p. 5 |
| Basic Theory of Ultracentrifugation | p. 7 |
| Svedberg's Simplified Theory | p. 8 |
| Derivation of Lamm's Equation | p. 10 |
| Basic Experiment Types of Ultracentrifugation | p. 12 |
| Sedimentation Velocity Experiment | p. 12 |
| Synthetic Boundary Experiment | p. 13 |
| Sedimentation Equilibrium | p. 13 |
| Density Gradient | p. 14 |
| Approach-to-Equilibrium (or Archibald) Method | p. 15 |
| Closing Remarks | p. 15 |
| References | p. 16 |
| Analytical Ultracentrifugation, Instrumentation | p. 17 |
| Ultracentrifuges | p. 18 |
| The Beckman-Coulter Optima XL-A/I | p. 19 |
| User-Made Centrifuges | p. 21 |
| Rotors | p. 25 |
| Measuring Cells | p. 26 |
| Detectors | p. 29 |
| Absorption Optics | p. 31 |
| Interference Optics | p. 34 |
| Schlieren Optics | p. 37 |
| Other Detectors | p. 38 |
| Multiplexer | p. 43 |
| Auxiliary Measurements | p. 43 |
| Measurement of the Solvent Density and the Partial Specific Volume | p. 43 |
| Measurement of the Refractive Index and the Specific Refractive Index Increment | p. 45 |
| References | p. 46 |
| Sedimentation Velocity | p. 47 |
| Introduction | p. 47 |
| Basic Example of Sedimentation Velocity | p. 47 |
| Determination of s | p. 50 |
| Standard Conditions for s Estimation | p. 51 |
| Radial Dilution and Thickening | p. 52 |
| Concentration Dependence | p. 53 |
| Advanced Theory of Sedimentation Velocity Runs | p. 54 |
| Johnston-Ogston Effect | p. 54 |
| Self-Sharpening of Boundarie | p. 55 |
| Pressure Dependence | p. 55 |
| Speed Dependence | p. 56 |
| Charge Effects | p. 57 |
| Separation of Sedimentation and Diffusion | p. 58 |
| Test of Homogeneity | p. 61 |
| Sedimentation Velocity Runs of Macromolecules to Measure Average M and MMD | p. 62 |
| Evaluation of the Average Molar Mass M by Sedimentation Velocity Runs via Scaling Laws | p. 62 |
| Evaluation of Molar Mass Distributions (MMD) by Sedimentation Velocity Runs via Scaling Laws | p. 64 |
| Sedimentation Velocity Runs on Particles to Measure Average d[subscript p] and PSD | p. 69 |
| Particle Size Distribution via AUC Turbidity Detector and Mie Theory | p. 70 |
| Coupling Technique to Measure very Broad PSD | p. 77 |
| H[subscript 2]0-D[subscript 2]0 Density Variation Method to Measure Particle Densities via Sedimentation Velocity Runs | p. 80 |
| PSD Measurement of very Small Platinum Clusters Using UV Optics | p. 84 |
| Synthetic Boundary Experiments | p. 86 |
| Synthetic Boundary Crystallization Ultracentrifugation | p. 93 |
| References | p. 95 |
| Density Gradients | p. 97 |
| Introduction | p. 98 |
| Static Density Gradients | p. 101 |
| Theory of Static Density Gradients | p. 102 |
| Gradient Materials | p. 115 |
| Experimental Procedure | p. 117 |
| Examples | p. 118 |
| Dynamic Density Gradients | p. 120 |
| Other Types of Density Gradients | p. 125 |
| Percoll Density Gradient | p. 125 |
| References | p. 129 |
| Sedimentation Equilibrium | p. 131 |
| Introduction | p. 131 |
| Experimental Procedure | p. 133 |
| Data Analysis | p. 138 |
| Basic Equations for Molar Mass Averages - Classical Evaluation of AUC Equilibrium Runs | p. 138 |
| Basic Equations for Molar Mass Distributions - Nonlinear Regression Evaluation (Lechner Method) | p. 141 |
| Application of the Basic Equations - Classical and Nonlinear Regression Evaluation | p. 144 |
| M-STAR, a Special Data Analysis to obtain M[subscript w] | p. 149 |
| Examples | p. 151 |
| Absorption Optics Examples of a Polyelectrolyte and Calibration Polystyrene NBS 706 | p. 152 |
| Association Equilibrium Example and AUC Buoyant Density Method to Determine [Characters not reproducible] | p. 155 |
| The New Fluorescence Detector of Laue Used for Green Fluorescent Protein | p. 158 |
| Further AUC Methods to Measure Molar Masses | p. 159 |
| References | p. 162 |
| Practical Examples of Combination of Methods | p. 163 |
| Combination of Different AUC Methods | p. 163 |
| Core/Shell Particles | p. 163 |
| Characterization of Microgels and Nanogels | p. 170 |
| Ion Exchange in Carboxylated Latices | p. 179 |
| Combination of AUC with Other Techniques | p. 189 |
| AUC and SFFF | p. 190 |
| AUC and EM | p. 198 |
| Literature Examples: AUC and Nanoparticles | p. 212 |
| References | p. 212 |
| Recent Developments and Future Outlook | p. 215 |
| New AUC Instrumentation and Detectors | p. 216 |
| New AUC Methods | p. 222 |
| New AUC Data Analysis | p. 228 |
| References | p. 233 |
| Subject Index | p. 235 |
| Table of Contents provided by Ingram. All Rights Reserved. |
