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Biotechnology Applications of Microinjection, Microscopic Imaging and Fluorescence

By: Peter H. Bach (Editor), C.H. Reynolds (Editor), J.M. Clark (Editor)

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Hardcover | 1 September 1993

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Hardcover
278 Pages

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24.41 x 16.99 x 1.75

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In 25 papers from a European workshop in London, England, April 1992, cell biologists explore how the microinjection of defined material can be used to manipulate the fundamental biochemistry of specific living cells. The techniques described tend to be highly sophisticated and highly expensive, so

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Microinjection: A Technique to Study Inhibition of HIV-1 Replication Mediated by Antisense RNA and Parvovirus genes	p. 1
How to Make Glass Microtools for the Injection of Isolated Plant Sperm Cells into Embryo Sac Cells Using a Microforge	p. 11
Application of the Firefly Luciferase Reporter Gene to Microinjection Experiments in Xenopus Oocytes	p. 19
Application of Confocal Microscopy for the Study of Neuronal Organization in Human Cortical Areas After Microinjection of Lucifer Yellow	p. 29
The Dye-Transfer Assay Permits the Evaluation of the Modulation of Junctional Communication by Chemicals: Results Obtained Employing a Highly Sensitive Video-Recording System Connected with the Microinjector	p. 37
Comparison of Fluorescent Dye Transfer in Intercellular Communication Studies Using Scrape-Loading and Microinjection Techniques	p. 45
Characterization of the Zea mays Embryo Sac Using Fluorescent Probes and Microinjection of Lucifer Yellow into the Female Cells	p. 53
Fluorescent Probes - Where Do They Go in Cells, and Why?	p. 61
Fluorescent Phospholipids in Membrane and Lipoprotein Research	p. 67
Selection of Fluorescent Golgi Complex Probes Using Structure-Activity Relationship Models	p. 73
Fluorescent Probes for the Assessment of the Subcellular Effects of Chemicals and Disease Processes In Vivo and In Vitro	p. 79
Whole-Cell Patch-Clamping: Introducing Substances into Cells During Electrical Measurements from the Cell Membrane: A Review of Potential Difficulties in Plant and Animal Cells	p. 99
Fluorescent Analysis of Replication and Intermediates of Chromatin Folding in Nuclei of Mammalian Cells	p. 111
Autofluorescence in Potato Tuber Phellum: Location and Time Course of Appearance During Tuber Formation	p. 121
Flow Cytometric Assessment of Gentamicin Nephrotoxicity in Established Renal Cell Lines	p. 129
The Use of the Fluorescent Probe Fura-2 for Intracellular Free Calcium Measurements: Some Methodological Aspects	p. 133
Studies on the Effects of Peroxisome Proliferators as Novel Ca[superscript 2+] Mobilizing Agents using Indo-1-Loaded Hepatocytes	p. 143
Confocal Fluorescence Ratio Imaging of pH in Plant Cells	p. 153
Analysis of the Calcium and Phosphorus Distribution in Predentine Tissue of Rat Incisors	p. 165
Quantitative Localisation of HIV Proteins in Mammalian Cells	p. 173
Concepts of the Cytometric Approach	p. 185
Laser Microperforation of Medicago sativa Root Hair Cells	p. 197
Application of pH-sensitive Fluorescent Dyes to Fungi	p. 207
Very Low Level Fluorescence Detection and Imaging Using a Long Exposure Charge Coupled Device System	p. 221
Modulation of Nucleus Formation in Mouse Oocytes Fused with Early and Late G2 Blastomeres	p. 231
Index	p. 239
Table of Contents provided by Blackwell. All Rights Reserved.

Biotechnology Applications of Microinjection, Microscopic Imaging and Fluorescence

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