Catalysts for Nitrogen Fixation

Preface to the Series. Preface. List of Contributors. 1: Nitrogen Fixation: An Historical Perspective; K. Fisher and W.E. Newton 1. Introduction 2. When did Biological Nitrogen Fixation Appear? 3. Nitrogen Fixation and Agriculture 4. Do Plants Assimilate Nitrogen from the Air? 5. Are Bacteria Responsible for Assimilating Nitrogen from the Air? 6. Do Free-Living Rhizobia Fix N2? 7. Commercial Application of Biological Nitrogen Fixation 8. Commercial Application of Industrial Nitrogen Fixation 9. Inorganic Ions and Nitrogen Fixation 10. Methods Used for the Detection of Nitrogen Fixation 11. Beginning of the Biochemistry of Biological Nitrogen Fixation 12. Cell-free Extracts and Beyond Acknowledgement References 2: Haber-Bosch and Other Industrial Processes; G.J. Leigh 1. Background to Industrial Fixation 2. Dinitrogen Chemistry up to ca. 1900 3. Industrial Fixation of Nitrogen 4. Developments since ca. 1920 5. Possible Future Developments References 3: Assay Methods for Products of Nitrogenase Action on Substrates; M.J. Dilworth 1. Introduction 2. Protons 3. HD Formation 4. Nitrogenous Substrates 5. Carbon-containing Substrates 6. Substrates containing Nitrogen and Carbon 7. Sulfur-containing Substrates 8. Other Assay Components 9. Concluding Remarks References 4: The Structures of the Nitrogenase Proteins and Stabilized Complexes; P.M.C. Benton and J.W. Peters 1. Introduction 2.The Fe Protein 3. The MoFe Protein 4. Nitrogenase Complex Structures Acknowledgements References 5: The Mechanism of Mo-dependent Nitrogenase: Thermodynamics and Kinetics; R.Y. Igarashi and L.C. Seefeldt 1. Introduction 2. The Fe-protein Cycle 3. The MoFe-protein Cycle 4. Future Prospects References 6: Strategies for the Functional Analysis of the Azotobacter vinelandii MoFe Protein and its Active Site FeMo-cofactor; S.M. Mayer, P.C. Dos Santos, L.C. Seefeldt and D.R. Dean 1. Introduction 2. Genetic Manipulation and Biochemical Techniques for the Study of A. vinelandii Nitrogenase 3. Insights gained into Nitrogenase Structure-Function from Genetic and Biochemical Studies 4. Summary and Outlook References 7: Chemical Models, Theoretical Calculations, and Reactivity of Isolated Iron-Molybdenum Cofactor; F. Barriere, M.C. Durrant and C.J. Pickett 1. Introduction 2. Chemical Models 3. Theoretical Calculations 4. Isolation and Reactivity of the Nitrogenase FeMo-cofactor 5. Summary and Future Prospects References 8: Structural Models for the FeMo-cofactor and the P Clusters; D.J. Evans 1. Introduction 2. FeMo-cofactor Models 3. The P Cluster 4. Concluding Remarks Acknowledgements References 9. Biosynthesis of Iron-Molybdenum and Iron-Vanadium Cofactors of the nif- and vnf-encoded Nitrogenases; P.W. Ludden, P. Rangaraj and L.M. Rubio 1. Introduction 2. Discovery and Characterization of FeMo-cofactor 3. Structures of FeMo-cofactor and FeV-cofactor and their Sites in the MoFe and VFe Proteins 4. FeMo-cofactor and FeV-cofactor Biosynthesis 5. in vitro FeMo-cofactor Synthesis 6. Role of NifQ 7. Role of NifB 8. Role of NifNE 9. Role NifH 10. NifV and the Role of Homocitrate 11. Role of NifX 12. Role of NifU 13. Role of NifS 14. Role of NifM 15. Roles of NifW and NifZ 16. Non-nif Protein Requirements 17. Role of VnfG 18. Role of Nucleotides and Divalent Metals in FeMo-cofactor Synthesis 19. Model for the Biosynthesis of FeMo-cofactor and FeV-cofactor References 10: Vanadium Nitrogenase; B.J. Hales 1. Introduction 2. Historical Background 3. Characterization 4. Mechanism 5. Genetics 6. Conclusions References 11. Iron-only Nitrogenase: Exceptional Catalytic, Structural and Spectroscopic Features; K. Schneider and A. Muller 1. Introduction 2. Metal Regulation of Nitrogenases 3. Factors Influencing Biosynthesis, Catalytic Activity, and Stability of Fe-nitrogenases 4. Mo- and Fe-nitrogenases: Comparison of Subunit Composition, Amino-acid Sequences and Immuno-reactions 5. Structures of the Iron-Sulfur Clusters in Fe-nitrogenase 6. EPR and Redox Properties of the Rhodobacter FeFe Protein 7. Catalytic Characteristics of Iron-only Nitrogenases 8. Fe-only Nitrogenase: Evolutionary Relic or Important Complementary Enzyme System for Diazotrophic Bacteria? 9. Summary and Outlook Acknowledgements References 12: Superoxide-dependent Nitrogenase; D. Gadkari 1. Introduction 2. Description of Streptomyces thermoautotrophicus 3. Components of the Superoxide-dependent Nitrogen-Fixing System 4. Reduction of N2 and Other Catalyzed reactions 5. Genetics 6. Conclusions and Perspectives References 13: Future Challenges and Prospects; R.L. Richards and B.E. Smith 1. Introduction 2. Challenges and Prospects 3. Conclusions References Subject Index.