Cell Cycle Checkpoint Control Protocols

Part I. Induction and Detection of Changes in Cell Cycle Progression Methods to Induce Cell Cycle Checkpoints Howard B. Lieberman and Kevin M. Hopkins Methods for Synchronizing Mammalian Cells Michael H. Fox Enrichment of Cells in Different Phases of the Cell Cycle by Centrifugal Elutriation Tej K. Pandita Analysis of the Mammalian Cell Cycle by Flow Cytometry Haiying Hang and Michael H. Fox Methods for Detecting Cells in S Phase Wei-Hsin Sun and Melvin L. DePamphilis Yeast Cell Synchronization Audra Day, Colette Schneider, and Brandt L. Schneider Analysis of the Budding Yeast Saccharomyces cerevisiae Cell Cycle by Morphological Criteria and Flow Cytometry Hong Zhang and Wolfram Siede Analysis of the Fission Yeast Schizosaccharomyces pombe Cell Cycle Eliana B. Gomez and Susan L. Forsburg Part II. Analysis of Genes Involved in Checkpoint Control Strategies to Isolate Evolutionarily Conserved Cell Cycle Regulatory Genes Scott Davey Microarray Approaches for Analysis of Cell Cycle Regulatory Genes Sally A. Amundson and Albert J. Fornace, Jr. Using the Yeast Genome-Wide Gene-Deletion Collection for Systematic Genetic Screens Jian Zhang, Lisa Ottmers, and Brandt L. Schneider Gene Targeting in Cultured Human Cells Todd A. Waldman Use of In Vivo Gap Repair for Isolation of Mutant Alleles of a Checkpoint Gene Migdalisel Colon and Nancy C. Walworth In Vitro Mutagenesis to Define Functional Domains Jian Qin, Zhe Peng, and Maureen V. McLeod Use of Gene Overexpression to Assess Function in Cell Cycle Control Erik K. Flemington and Antonio Rodriguez Histone Acetylation/Deacetylation As a Regulator of Cell Cycle Gene Expression Chenguang Wang, Maofu Fu, and Richard G. Pestell Part III. Analysis of Proteins Involved in Checkpoint Control Cataloging Proteins in Cell Cycle Control Kazimierz O. Wrzeszczynski and Burkhard Rost Multidimensional Proteomic Analysis of Proteolytic Pathways Involved in Cell Cycle Control Michael W. Schmidt, Aruna Jain, and Dieter A. Wolf Purification and Identification of Protein Complexes That Control the Cell Cycle Matthew A. Burtelow, Vladimir N. Podust, and Larry M. Karnitz Xenopus Cell-Free Extracts to Study DNA Damage Checkpoints Vincenzo Costanzo and Jean Gautier Protein-Protein Interactions Graziella Pedrazzi and Igor Stagljar Detection of Kinase and Phosphatase Activities Sean M. Post and Eva Y.-H.P. Lee Monitoring Changes in the Subcellular Location of Proteins in S. cerevisiae Diego Rua, Teresa Holzen, Benjamin S. Glick, Stephen J. Kron, and Douglas K. Bishop Part IV. Chromosomes and the Cell Cycle Chromosomal Changes and Cell Cycle Checkpoints in Mammalian Cells Charles R. Geard and Brian Ponnaiya Detecting the Influence of Cell Cycle Regulatory Proteins on Human Telomeres Tej K. Pandita Monitoring Spindle Assembly and Disassembly in Yeast by Indirect Immunofluorescence Rita K. Miller Index