Part I. Special Reviews The Temporal Sequence of Changes in Gene Expression by Drugs of Abuse Peter W. Kalivas, Shigenobu Toda, M. Scott Bowers, David A. Baker, and M. Behnam Ghasemzadeh Effects of Psychomotor Stimulants on Glutamate Receptor Expression Marina E. Wolf Adult Neural Stem/Progenitor Cells in the Forebrain: Implications for Psychostimulant Dependence and Medication John Q. Wang, Limin Mao, and Yuen-Sum Lau Neuroprotective Effect of Naloxone in Inflammation-Mediated Dopaminergic Neurodegeneration: Dissociation from the Involvement of Opioid Receptors Bin Liu and Jau-Shyong Hong Neuropharmacology and Neurotoxicity of 3,4-Methylenedioxymethamphetamine Gary A. Gudelsky and Bryan K. Yamamoto Learning and Memory Mechanisms Involved in Compulsive Drug Use and Relapse Joshua D. Berke From Drugs of Abuse to Parkinsonism: The MPTP Mouse Model of Parkinson's Disease Yuen-Sum Lau and Gloria E. Meredith Part II. Detection of mRNA Expression in the Striatum In Situ Hybridization with Isotopic Riboprobes for Detection of Striatal Neuropeptide mRNA Expression After Dopamine Stimulant Administration Yasmin L. Hurd Combining In Situ Hybridization with Retrograde Tracing and Immunohistochemistry for Phenotypic Characterization of Individual Neurons Catherine Le Moine Analysis of mRNA Expression Using Double In Situ Hybridization Labeling with Isotopic and Nonisotopic Probes John Q. Wang Quantification of mRNA in Neuronal Tissue by Northern Analysis Christine L. Konradi Analysis of Gene Expression in Striatal Tissue by Multiprobe RNase Protection Assay Neil M. Richtand Analysis of mRNA Expression in Striatal Tissue by Differential Display Polymerase Chain Reaction Joshua D. Berke Semiquantitative Real-Time PCR for Analysis of mRNA Levels Stephen J. Walker, Travis J. Worst, and Kent E. Vrana Application of TaqMan RT-PCR for Real-Time Semiquantitative Analysis of Gene Expression in the Striatum Andrew D. Medhurst and Menelas N. Pangalos Systematic Screening of Gene Expression Using a cDNA Macroarray Travis J. Worst, Willard M. Freeman, Stephen J. Walker, and Kent E. Vrana Part III. Detection of Protein Expression in the Striatum Quantification of Protein in Brain Tissue by Western Immunoblot Analysis Christine L. Konradi Immunohistochemical and Immunocytochemical Detection of Phosphoproteins in Striatal Neurons Limin Mao and John Q. Wang Analysis of Protein Expression in Brain Tissue by ELISA Steffany A. L. Bennett and David C. S. Roberts Dopamine Receptor Binding and Quantitative Autoradiographic Study Beth Levant Analysis of DNA-Binding Activity in Neuronal Tissue with the Electrophoretic Mobility-Shift Assay Christine L. Konradi Part IV. Gene Function Analysis Viral-Mediated Gene Transfer to Study the Behavioral Correlates of CREB Function in the Nucleus Accumbens of Rats William A. Carlezon, Jr. and Rachael L. Neve Generating Gene Knockout Mice for Studying Mechanisms Underlying Drug Addiction Jianhua Zhang and Ming Xu Antisense Approaches in Analyzing the Functional Role of Proteins in the Central Nervous System John Q. Wang and Limin Mao Part V. Striatal Culture Preparation Primary Striatal Neuronal Culture Limin Mao and John Q. Wang Primary Rat Mesencephalic Neuron-Glia, Neuron-Enriched, Microglia-Enriched, and Astroglia-Enriched Cultures Bin Liu and Jau-Shyong Hong Primary Culture of Adult Neural Progenitors Steffany A. L. Bennett and Lysanne Melanson-Drapeau Organotypic Culture of Developing Striatum: Pharmacological Induction of Gene Expression Fu-Chin Liu Part VI. Detection of Transmitter Release in the Striatum Microdialysis Coupled with Electrochemical Detection: A Way to Investigate Brain Monoamine Role in Freely Moving Animals Ezio Carboni HPLC/EC Detection and Quantification of Acetylcholine in Dialysates James A. Zackheim and Elizabeth D. Abercrombie Real-Time Measurements of Phasic Changes in Extracellular Dopamine Concentration in Freely Moving Rats by Fast-Scan Cyclic Voltammetry Paul E. M. Phillips, Donita L. Robinson, Garret D. Stuber, Regina M. Carelli, and R. Mark Wightman Measurement of Dopamine Uptake in Neuronal Cells and Tissues Yuen-Sum Lau Part VII. Behavioral Assessment and Others Laboratory Analysis of Behavioral Effects of Drugs of Abuse in Rodents Neil M. Richtand Conditioned Place Preference: A Simple Method for Investigating Reinforcing Properties in Laboratory Animals Ezio Carboni and Cinzia Vacca Detection of Cell Proliferation and Cell Fate in Adult CNS Using BrdU Double-Label Immunohistochemistry Zin Z. Khaing and Mariann Blum Index
