Enzyme Engineering

1. A Tripartite Fusion System for the Selection of Protein Variants with Increased Stability in vivoLinda Foit and James Bardwell 2. Determining Enzyme Kinetics via Isothermal Titration CalorimetryNeil A. Demarse, Marie C. Killian, Lee D. Hansen, and Colette F. Quinn 3. GFP Reporter Screens for the Engineering of Amino Acid Degrading Enzymes from Libraries Expressed in BacteriaOlga Paley, Giulia Agnello, Jason Cantor, Tae Hyun Yoo, George Georgiou, and Everett Stone 4. Flow Cytometric Assays for Interrogating LAGLIDADG Homing Endonuclease DNA Binding and Cleavage PropertiesSarah K. Baxter, Abigail R. Lambert, Andrew M. Scharenberg, and Jordan Jarjour 5. TAL Effector Nuclease (TALEN) EngineeringTing Li and Bing Yang 6. In vitro Evolution of EnzymesMisha V. Golynskiy, John C. Haugner III, Aleardo Morelli, Dana Morrone, and Burckhard Seelig 7. Residue-Specific Incorporation of Unnatural Amino Acids into Proteins In vitro and In vivoAmrita Signh-Blom, Randall A. Hughes, and Andrew. D. Ellington 8. Reconstructing Evolutionary Adaptive Paths for Protein EngineeringMegan F. Cole, Vanessa E. Cox, Kelsey L. Gratton, and Eric A. Gaucher 9. Oligonucleotide Recombination Enabled Site-Specific Mutagenesis in BacteriaBryan M. Swingle 10. FX Cloning: A Versatile High-Throughput Cloning System for Characterization of Enzyme Variants Eric R. Geertsma 11. Use of Sulfolobus solfataricus PCNA Subunit Proteins to Direct the Assembly of Multimeric Enzyme ComplexesHidehiko Hirakawa and Teruyuki Nagamune 12. Gene Synthesis by Assembly of Deoxyuridine Containing Oligonucleotides Romualdas Vaisvila and Jurate Bitinaite 13. Protein Engineering: Single or Multiple Site-Directed MutagenesisPei-Chung Hsieh and Romualdas Vaisvila 14. Gene Assembly and Combinatorial Libraries in S. cerevisiae via Reiterative RecombinationNili Ostrov, Laura M. Wingler, and Virginia Cornish 15. Promiscuity-Based Enzyme Selection for Rational Directed Evolution ExperimentsSandeep Chakraborty, Renu Minda, Lipika Salaye, Abhaya M. dandekar, Swapan K. Bhattacharjee, and Basuthkar J. Rao 16. Rational Protein Sequence Diversification by Multi-Codon Scanning MutagenesisJia Liu and T. Ashton Cropp 17. Screening Libraries for Improved Solubility: Using E. coli Dihydrofolate Reductase as a ReporterJian-Wei Liu and David Ollis 18. In Vitro Directed Evolution of Enzymes Expressed by E. coli in Micro-Titre Plates Bradley J. Stevenson, Sylvia H.C. Yip, and David L. Ollis