1. A Tripartite Fusion System for the Selection of Protein Variants with Increased Stability in vivo
Linda Foit and James Bardwell
2. Determining Enzyme Kinetics via Isothermal Titration Calorimetry
Neil A. Demarse, Marie C. Killian, Lee D. Hansen, and Colette F. Quinn
3. GFP Reporter Screens for the Engineering of Amino Acid Degrading Enzymes from Libraries Expressed in Bacteria
Olga Paley, Giulia Agnello, Jason Cantor, Tae Hyun Yoo, George Georgiou, and Everett Stone
4. Flow Cytometric Assays for Interrogating LAGLIDADG Homing Endonuclease DNA Binding and Cleavage Properties
Sarah K. Baxter, Abigail R. Lambert, Andrew M. Scharenberg, and Jordan Jarjour
5. TAL Effector Nuclease (TALEN) Engineering
Ting Li and Bing Yang
6. In vitro Evolution of Enzymes
Misha V. Golynskiy, John C. Haugner III, Aleardo Morelli, Dana Morrone, and Burckhard Seelig
7. Residue-Specific Incorporation of Unnatural Amino Acids into Proteins In vitro and In vivo
Amrita Signh-Blom, Randall A. Hughes, and Andrew. D. Ellington
8. Reconstructing Evolutionary Adaptive Paths for Protein Engineering
Megan F. Cole, Vanessa E. Cox, Kelsey L. Gratton, and Eric A. Gaucher
9. Oligonucleotide Recombination Enabled Site-Specific Mutagenesis in Bacteria
Bryan M. Swingle
10. FX Cloning: A Versatile High-Throughput Cloning System for Characterization of Enzyme Variants
Eric R. Geertsma
11. Use of Sulfolobus solfataricus PCNA Subunit Proteins to Direct the Assembly of Multimeric Enzyme Complexes
Hidehiko Hirakawa and Teruyuki Nagamune
12. Gene Synthesis by Assembly of Deoxyuridine Containing Oligonucleotides
Romualdas Vaisvila and Jurate Bitinaite
13. Protein Engineering: Single or Multiple Site-Directed Mutagenesis
Pei-Chung Hsieh and Romualdas Vaisvila
14. Gene Assembly and Combinatorial Libraries in S. cerevisiae via Reiterative Recombination
Nili Ostrov, Laura M. Wingler, and Virginia Cornish
15. Promiscuity-Based Enzyme Selection for Rational Directed Evolution Experiments
Sandeep Chakraborty, Renu Minda, Lipika Salaye, Abhaya M. dandekar, Swapan K. Bhattacharjee, and Basuthkar J. Rao
16. Rational Protein Sequence Diversification by Multi-Codon Scanning Mutagenesis
Jia Liu and T. Ashton Cropp
17. Screening Libraries for Improved Solubility: Using E. coli Dihydrofolate Reductase as a Reporter
Jian-Wei Liu and David Ollis
18. In Vitro Directed Evolution of Enzymes Expressed by E. coli in Micro-Titre Plates
Bradley J. Stevenson, Sylvia H.C. Yip, and David L. Ollis
