| Fluorescence Microscopy: State of the Art | p. 1 |
| Fluorescence Lifetime-Resolved Imaging Microscopy: A General Description of LIfetime-Resolved Imaging Measurements | p. 15 |
| Confocal Fluorescence Lifetime Imaging | p. 35 |
| Multidimensional Fluorescence Microscopy: Optical Distortions in Quantitative Imaging of Biological Specimens | p. 47 |
| Fluorescent Probes | p. 57 |
| Flow Cytometry versus Fluorescence Microscopy | p. 61 |
| Multichannel Fluorescence Microscopy and Digital Imaging: On the Exciting Developments in Fluorescence Microscopy | p. 67 |
| Fluorescence Lifetime Imaging and Spectroscopy in Photobiology and Photomedicine | p. 71 |
| A Versatile Time-Resolved Laser Scanning Confocal Microscope | p. 79 |
| Disappearance of Cytoplasmic Ca[superscript 2+] Oscillations Is a Sensitive Indicator of Photodamage in Pancreatic [beta]-Cells | p. 85 |
| Distribution of Individual Cytoplasmic pH Values in a Cell Suspension | p. 91 |
| The Effect of Lysosomal pH on Lactoferrin-Dependent Iron Uptake in Tritrichomonas foetus | p. 95 |
| On the Protein-Error of the Calcium-Sensitive Fluorescent Indicator Fura-Red | p. 101 |
| Cytoplasmic Ion Imaging: Evidence for Intracellular Calibration Heterogeneities of Ion-Sensitive Flourophrobes | p. 107 |
| The Effect of Protein Binding on the Calibration Curve of the pH Indicator BCECF | p. 113 |
| Artifacts in Fluorescence Ratio Imaging | p. 119 |
| Use of Fluorescent Probes and CLSM for pH-Monitoring in the Whole Plant Tissue: pH Changes in the Shoot Apex of Chenopodium rubrum Related to Organogenesis | p. 125 |
| Spatial Resolution of Cortical Cerebral Blood Flow and Brain Intracellular pH as Measured by in Vivo Fluorescence Imaging | p. 133 |
| Is a Potential-Sensitive Probe diS-c[subscript 3](3) a Nernstian Dye?: Time-Resolved Fluorescence Study with Liposomes as a Model System | p. 139 |
| Kinetic Behavior of Potential-Sensitive Fluorescent Redistribution Probes: Modelling of the Time Course of Cell Staining | p. 145 |
| Speed of Accumulation of the Membrane Potential Indicator dis-c3(3) in Yeast Cells | p. 151 |
| Spectral Effects of Slow Dye Binding to Cells and Their Role in Membrane Potential Measurements | p. 157 |
| Exploitation of Rhodamine B in the Killer Toxin Research | p. 163 |
| "In Situ" Estimates of the Spatial Resolution for "Practical" Fluorescence Microscopy of Cell Nuclei | p. 169 |
| Requirements for a Computer-Based System for FISH Applications | p. 175 |
| Fluorescent Dyes and Dye Labelled Probes for Detection of Nucleic Acid Sequences in Biological Material | p. 179 |
| Fluorescence in Situ Hybridization (FISH) in Cytogenetics of Leukemia | p. 185 |
| Estimation of "Start" in Saccharomyces cerevisiae by Flow Cytometry and Fluorescent Staining of DNA and Cell Protein | p. 191 |
| Fluorescence Image Cytometry of DNA Content: A Comparative Study of Three Fluorochromes and Four Fixation Protocols | p. 197 |
| In Vivo Tissue Characterization Using Environmentally Sensitive Fluorochromes | p. 203 |
| Sensitive and Rapid Detection of [beta]-Galactosidase Expression in Intact Cells by Microinjection of Fluorescent Substrate | p. 211 |
| Fluorogenic Substrates Reveal Genetic Differences in Aldehyde-Oxidating Enzyme Patterns in Rat Tissues | p. 217 |
| Binding of Prothrombin Fragment 1 to Phosphatidylserine Containing Vesicles: A Solvent Relaxation Study | p. 223 |
| New Thiol Active Fluorophores for Intracellular Thiols and Glutathione Measurement | p. 229 |
| Quantification of Macrophages in the Cardiovascular System of Hypercholesterolemic Rabbits by Use of Digital Image Processing | p. 235 |
| Fluorescence Assay for Studying P-Glycoprotein Function at Single Cell Level | p. 241 |
| Alterations of Vimentin-Nucleus Interactions as an Early Phase in Cholesterol Oxide-Induced Endothelial Cell Damage | p. 247 |
| Fluorescence Microscopy of Rye Cell Walls from Kernels to Incubated Doughs | p. 253 |
| Practical Approach for Immunohistochemical Staining of Muscle Biopsies | p. 257 |
| Rapid Automatic Segmentation of Fluorescent and Phase-Contrast Images of Bacteria | p. 261 |
| Use of Confocal Microscopy for Absolute Measurement of Cell Volume and Total Cell Surface Area | p. 267 |
| Cell Volume Measurements Using Confocal Laser Scanning Microscopy | p. 273 |
| Subcellular Cytofluorometry in Confocal Microscopy | p. 279 |
| Application of Confocal Microscopy to 3-D Reconstruction and Morphometrical Analysis of Capillaries | p. 285 |
| Retrieving Spatio Temporal Information from Confocal Data: A Study Using Melanotrope Cells of Xenopus laevis | p. 291 |
| Dynamics of Actin Measured by Fluorescence Correlation Microscopy (FCM) | p. 297 |
| Index | p. 303 |
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