| Introduction | p. 1 |
| References for Part I | p. 3 |
| General Principles of Enzyme Detection on Electrophoretic Gels | p. 5 |
| Chromogenic Reactions | p. 5 |
| Products Reducing Tetrazolium Salts | p. 5 |
| Products Capable of Coupling with Diazonium Salts | p. 6 |
| Products that Cause a pH Change | p. 7 |
| Orthophosphate | p. 8 |
| Pyrophosphate | p. 9 |
| Hydrogen Peroxide | p. 9 |
| Carbonate Ions | p. 10 |
| Colored Products | p. 10 |
| Products Bearing Reduced Thiol Groups | p. 10 |
| Products that Influence the Starch-Iodine Reaction | p. 11 |
| Products of Polymerizing Enzymes | p. 11 |
| Products of Depolymerizing Enzymes | p. 11 |
| Fluorogenic Reactions | p. 12 |
| NADH and NADPH | p. 12 |
| 4-Methylumbelliferone | p. 12 |
| Products that Form Luminescent Lanthanide Chelates | p. 13 |
| Miscellany | p. 13 |
| Autoradiography | p. 13 |
| Bioautography | p. 14 |
| Two-Dimensional Gel Spectroscopy | p. 14 |
| Immunoblotting | p. 15 |
| Immobilization Matrices | p. 15 |
| Transfer of Proteins | p. 16 |
| Specific Antibodies and Labeled Anti-Antibodies | p. 17 |
| Detection of Transferred Proteins on Blots | p. 17 |
| Miscellanies | p. 18 |
| Detection by Incorporating Water-Insoluble Substrates into Separating Gels | p. 18 |
| Detection of Lipid-Metabolizing Enzymes | p. 18 |
| Detection by a Two-Dimensional Electrophoresis Procedure | p. 18 |
| Detection of Enzymatic Proteins by Specific Probes | p. 19 |
| Labeled Aptamers and Aptamer Beacons as Specific Probes | p. 19 |
| Labeled Inhibitors as Specific Probes | p. 19 |
| Specific Detection of Some Nonenzymatic Proteins | p. 20 |
| Detection of Proteinase and Ribonuclease Inhibitory Proteins Using Reverse Zymography | p. 20 |
| Detection of Proteinase Inhibitory Proteins Using Biotinylated Proteinases as Specific Probes | p. 20 |
| Biotinylated Hyaluronan as a Specific Probe for Hyaluronan-Binding Proteins | p. 20 |
| Transferrin | p. 20 |
| References for Part II | p. 20 |
| Methods of Detection of Specific Enzymes | p. 23 |
| The Structure of Enzyme Sheets | p. 23 |
| References | p. 25 |
| General Considerations, Comments, and Recommendations | p. 25 |
| The Choice of Support Medium for Enzyme Electrophoresis and Detection | p. 25 |
| Cellulose Acetate Gel | p. 25 |
| Polyacrylamide Gel | p. 26 |
| Starch Gel | p. 27 |
| Strategies of Gel Staining | p. 27 |
| The Choice of Zymogram Method | p. 28 |
| Preparation of Staining Solution | p. 28 |
| Modes of Application of Staining Solutions | p. 29 |
| Modes of Enhancement of Staining Intensity of Enzyme Activity Bands | p. 30 |
| Specificity of Zymogram Methods and Some Related Problems | p. 31 |
| Recording and Preservation of Zymograms | p. 33 |
| Resource-Saving Strategies | p. 34 |
| Simultaneous Detection of Several Enzymes on the Same Gel | p. 34 |
| Successive Detection of Different Enzymes on the Same Gel | p. 35 |
| The Reuse of Staining Solutions | p. 36 |
| The Use of Several Origins on One Gel | p. 36 |
| Multiple Replication of Electrophoretic Gels by Electroblotting | p. 36 |
| The Use of Semipreparative Gels for Production of Preparations of Linking Enzymes | p. 36 |
| Troubleshooting | p. 37 |
| Safety Regulations | p. 37 |
| References | p. 38 |
| Enzyme Sheets* | p. 41 |
| Minimal Medium for Escherichia coli | p. 541 |
| Citrate Medium for Pediococcus cerevisiae | p. 542 |
| Alphabetical List of Enzymes | p. 544 |
| Buffer Systems Used for Enzyme Electrophoresis | p. 549 |
| Table of Contents provided by Syndetics. All Rights Reserved. |
