Preface | p. v |
Contributors | p. xi |
General Techniques | |
Proteomics and the Molecular Biologist | p. 3 |
Protein Identification from 2-D Gels Using In Vitro Transcription Translation Products | p. 17 |
Selective Chemical Cleavage Methods in Proteomics, Including C-Terminal Successive Degradation | p. 29 |
Means of Hydrolyzing Proteins Isolated upon ProteinChip Array Surfaces: Chemical and Enzymatic Approaches | p. 59 |
A Combined Radiolabeling and Silver Staining Technique for Improved Visualization and Localization of Proteins on Two-Dimensional Gels | p. 73 |
Qualitative and Quantitative Proteomic Analyses via Multidimensional Protein Identification Technology | p. 79 |
Di- and Tri-Chromatic Fluorescence Detection on Western Blots | p. 93 |
Multiplexed Proteomics: Fluorescence Detection of Protein Differences by Two-Dimensional Gel Electrophoresis and Computer-Based Differential Display | p. 107 |
A Strategy for Characterizing Antibody/Antigen Interactions Using ProteinChip Arrays | p. 117 |
Stable Isotope Labeling with Amino Acids as an Aid to Protein Identification in Peptide Mass Fingerprinting | p. 129 |
The Use of [superscript 18]O Labeling as a Tool for Proteomic Applications | p. 145 |
Automated Nanoflow Liquid Chromatography/Tandem Mass Spectrometric Identification of Liver Mitochondrial Proteins | p. 181 |
In Silico Proteomics: Predicting Interactions from Sequence | p. 193 |
Post-Translational Modifications, Variants, and Isoforms | |
Predicting Glycan Composition from Experimental Mass Using GlycoMod | p. 225 |
Querying GlycoSuiteDB | p. 233 |
New Tools for Quantitative Phosphoproteome Analysis | p. 241 |
Computer-Aided Strategies for Characterizing Protein Isoforms | p. 259 |
Protein Variant Separations Using Cation Exchange Chromatography on Grafted, Polymeric Stationary Phases | p. 269 |
Specific Systems | |
Noninvasive Imaging of Protein-Protein Interactions in Living Animals | p. 283 |
Strategies in Clinical Proteomics | p. 299 |
Proteomic Profiling of the Cancer Microenvironment | p. 309 |
Identification of Determinants of Sensitivity to Antitumor Drugs | p. 319 |
Application of Proteomics to the Discovery of Serological Tumor Markers | p. 331 |
Infectomic Analysis of Microbial Infections Using Proteomics | p. 347 |
Toward a Complete Proteome of Bacillus subtilis: Cytosolic, Cell Wall-Associated, and Extracellular Proteins | p. 357 |
Renal and Urinary Proteomics | p. 375 |
Proteomics in Endocrinology | p. 397 |
Proteomics in Plant Biology | p. 409 |
Data Analysis | |
Bioinformatics in Proteomics | p. 419 |
Quantitative Characterization of Proteomics Maps by Matrix Invariants | p. 429 |
Complexity of Protein-Protein Interaction Networks, Complexes, and Pathways | p. 451 |
Patchwork Peptide Sequencing: Protein Identification by Accurate Mass-to-Sequence Conversion of High-Resolution Q-TOF Tandem Mass Spectrometry Data | p. 463 |
Estimation of Bias in Proteome Research | p. 473 |
Scoring Functions for Mass Spectrometric Protein Identification | p. 477 |
Index | p. 487 |
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