Heterologous Expression of Membrane Proteins

1. Cell-Free Production of Membrane Proteins in Escherichia coli Lysates for Functional and Structural Studies Ralf-Bernhardt Rues, Erik Henrich, Coilin Boland, Martin Caffrey, and Frank Bernhard 2. Membrane Protein Production in the Yeast, S. cerevisiae Stephanie P. Cartwright, Lina Mikaliunaite, and Roslyn M. Bill 3. Membrane Proteins Production in Escherichia coli: Protocols and Rules Federica Angius, Oana Ilioaia, Marc Uzan, and Bruno Miroux 4. Codon Optimizing for Increased Membrane Protein Production: A Minimalist Approach Kiavash Mirzadeh, Stephen Toddo, Morten H.H. Norholm, and Daniel O. Daley 5. Generation of Tetracycline-Inducible Mammalian Cell Lines by Flow Cytometry for Improved Overproduction of Membrane Proteins Juni Andrell, Patricia C. Edwards, Fan Zhang, Maria Daly, and Christopher G. Tate 6. Membrane Protein Production in Lactococcus lactis for Functional Studies Daphne Seigneurin-Berny, Martin S. King, Emiline Sautron, Lucas Moyet, Patrice Catty, Francois Andre, Norbert Rolland, Edmund R.S. Kunji, and Annie Frelet-Barrand 7. Expression of Viral Envelope Glycoproteins in Drosophila melanogaster S2 Cells Renato Mancini Astray, Sandra Fernanda Suarez-Patino, and Soraia Attie Calil Jorge 8. Leishmania tarentolae as a Promising Tool for Expressing Polytopic and Multi-Transmembrane Spans Eukaryotic Membrane Proteins: The Case of the ABC Pump ABCG6 Lucia Gonzalez-Lobato, Vincent Chaptal, Jennifer Molle, and Pierre Falson 9. Overexpression, Membrane Preparation, and Purification of a Typical Multidrug ABC Transporter BmrA Benjamin Wiseman and Jean-Michel Jault 10. Expression of Eukaryotic Membrane Proteins in Pichia pastoris Lucie Hartmann, Valerie Kugler, and Renaud Wagner 11. Integral Membrane Protein Expression in Saccharomyces cerevisiae Rebba C. Boswell-Casteel, Jennifer M. Johnson, Robert M. Stroud, and Franklin A. Hays 12. High-Throughput Baculovirus Expression System for Membrane Protein Production Ravi C. Kalathur, Marinela Panganiban, and Renato Bruni 13. Small-Scale Screening to Large-Scale Over-Expression of Human Membrane Proteins for Structural Studies Sarika Chaudhary, Sukanya Saha, Sobrahani Thamminana, and Robert M. Stroud 14. Purification of Human and Mammalian Membrane Proteins Expressed in Xenopus laevis Frog Oocytes for Structural Studies Rajendra Boggavarapu, Stephan Hirschi, Daniel Harder, Marcel Meury, Zohre Ucurum, Marc J. Bergeron, and Dimitrios Fotiadis 15. <membrane protein=" solubilization=" and=" composition=" of=" detergent=" complexesmembrane=" complexesKatia Duquesne, Valerie Prima, and James N. Sturgis 16. Detergent-Free Membrane Protein Purification Alice J. Rothnie 17. Conformational Dynamics and Interactions of Membrane Proteins by Hydrogen/Deuterium Mass Spectrometry Eric Forest and Petr Man 18. Lessons from an a-Helical Membrane Enzyme: Expression, Purification, and Detergent Optimization for Biophysical and Structural Characterization Jennifer L. Johnson, Sibel Kalyoncu, and Raquel L. Lieberman 19. Method to Screen Multidrug Transport Inhibitors Using Yeast Over-Expressing a Human MDR Transporter Laura Fiorini and Isabelle Mus-Veteau