In Situ Hybridization in Light Microscopy

By: Gerard Morel, Annie Cavalier

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Hardcover | 28 September 2000 | Edition Number 1

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Hardcover
342 Pages

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25.4 x 17.78 x 2.06

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In Situ hybridization allows the visualization of specific DNA/RNA sequences in individual cells in tissue sections, single cells, or chromosome preparations, and is an especially important method for studying DNA and RNA in heterogeneous cell populations. This book delves into in situ hybridization methods through the use of light microscopy used by molecular biologists, pathologists, geneticists, and biochemists. It will also appeal to research scientists who are interested in visualizing methods for nucleic acids and proteins. The book features a two-column layout for protocols/principles, simplifies the techniques and offers many schemes and tables to help the reader choose the best procedure.

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General Introduction	p. vii
Abbreviations	p. xiii
Probes	p. 1
Tissue Preparation	p. 61
Pretreatments	p. 89
Hybridization	p. 107
Revelation of Hybrids	p. 139
Counterstaining and Modes of Observation	p. 187
Controls and Problems	p. 207
Typical Protocols	p. 221
Examples of Observations	p. 251
Appendices	p. 261
Equipment	p. 267
Reagents	p. 273
Glossary	p. 303
Index	p. 319
Table of Contents provided by Syndetics. All Rights Reserved.

In Situ Hybridization in Light Microscopy

At a Glance

Hardcover

Shipping

How to return your order

Defective items

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