Introduction to Luminescence Assay | p. 1 |
Light Probes | p. 3 |
Instrumentation for the Measurement of Luminescence | p. 31 |
Calculations to Quantify Luminescent Measurements | p. 41 |
A Low-Budget Luminometer for Sensitive Chemiluminescent Immunoassays | p. 47 |
Functionality of Commercially Available Instruments for Luminescence | p. 55 |
Survey of Commercially Available Instruments for Luminescence | p. 69 |
Light Probes/Clinical Assay | p. 75 |
Chemiluminescent N-Sulfonylacridinium-9-Carboxamides and Their Application in Clinical Assays | p. 77 |
Bioluminescence Detection of Proteolytic Bond Cleavage by Using Recombinant Aequorin | p. 107 |
Development of New Label Enzymes for Bioluminescent Enzyme Immunoassay | p. 121 |
Assay of Diversified Biomolecules with a Luminogenic Conjugate Substrate: 5-(5'Azoluciferinyl)-2,3-Dihydro-1,4-Phthalazinedione | p. 131 |
Hybrid Capture: A System for Nucleic Acid Detection by Signal Amplification Technology | p. 149 |
Molecular Biological Applications of Luminescence | p. 159 |
Chemiluminescent Technology: Optimization of Western Blots and ELISAs Utilizing a Horseradish Peroxidase Label in Automated Systems | p. 161 |
A Luminol/Iodophenol Chemiluminescent Detection System for Western Immunoblots | p. 179 |
Chemiluminescent Detection of Immobilized Nucleic Acids--From Southern Blots to Microarrays | p. 189 |
Differential Display Polymerase Chain Reaction Using Chemiluminescent Detection | p. 203 |
Nonradioactive Labeled Amplified Fragment Length Polymorphisms for Application in Forest Trees | p. 211 |
Detection of Nucleic Acids Using Chemiluminescence: From Northerns to Southerns and Beyond | p. 223 |
Luciferase Assay: A Powerful Tool to Determine Toxic Metal-Induced NF-[kappa]B Activation | p. 231 |
Pyrosequencing: A Bioluminometric Method of DNA Sequencing | p. 247 |
Pathway-Specific cDNA Array Using Luminescent Detection | p. 255 |
Cellular Luminescence | p. 259 |
Application of Chemiluminescence in Phagocyte-Pathogen Interactions | p. 261 |
L-012: An Ultrasensitive Reagent for Detecting Cellular Generation of Reactive Oxygen Species | p. 279 |
Avoiding the Jaws of Using Lucigenin as a Chemiluminigenic Probe for Biological Superoxide: Determining When It's Safe to Go Back into the Water | p. 287 |
Chemiluminescent Polymer Microspheres for Measuring Reactive Oxygen Species | p. 305 |
The Effect of Antibiotics on Phagocytic Function of Granulocytes | p. 321 |
In Vitro CMI: Rapid Assay for Measuring Cell-Mediated Immunity | p. 331 |
Protective Effects of Native but Not Oxidized High-Density Lipoprotein against Proinflammatory Respiratory Burst Activities of Polymorphonuclear Leukocytes Induced by Hypochlorite-Oxidized Low-Density Lipoprotein | p. 345 |
Peroxynitrite-Based Luminol Luminescence of Macrophages and Enhancement of the Signal | p. 365 |
Oxidative Stress | p. 379 |
Overview of Oxidative Stress | p. 381 |
Luminescence Analysis of Oxidative Stress Induced by Organ Transplantation | p. 385 |
Acceleration of Tissue Aging in Chickens Caused by Oxidative Stress Using Allopurinol and Detected by Cellular Humoral Chemiluminescence | p. 393 |
Enhancement of Luminol-Dependent Peroxynitrite Luminescence in Dishes and Tubes from Various Macrophages: Rat Alveolar Macrophages Apparently Display a New Oxidative Mechanism | p. 409 |
A Search to Detect Antioxidants Using Luminescence Useful for Treating Oxidative Stress | p. 417 |
Luminol Luminescence Generated by a Novel Peroxynitrite Mechanism Is Inhibited by Polyphenolic Plant Extracts: Nitroglycerin Tablets and Superoxide Generate Peroxynitrite | p. 429 |
Antioxidant Activity in Grape and Other Fruit Extracts Inhibits Peroxynitrite-Dependent Oxidation (from SIN-1) as Measured by Luminescence | p. 435 |
Antioxidant Actions of Acetaminophen Preventing Myocardial Injury Detected by Luminescence and Other Modalities | p. 443 |
Luciferase Luminescence Can Be Used to Access Oxidative Damage to Plasmid DNA and Its Prevention by Selected Fruit Extracts | p. 459 |
Luminescent Imaging | p. 467 |
Detection Systems Optimized for Low-Light Chemiluminescence Imaging | p. 469 |
Applications of Bioluminescent and Chemiluminescent Imaging in Analytical Biotechnology | p. 481 |
Quantification of the [alpha;[subscript 3] Subunit of the Na[superscript +]/K[superscript +]-ATPase in Cerebellar Purkinje Neurons Using Luminescence | p. 503 |
The Influence of Plates on Luminescence Measurement | p. 511 |
Whole-Body Bioluminescent Imaging for the Study of Animal Models of Human Bacterial Disease | p. 517 |
High-Throughput Screening | p. 527 |
Resonance Energy Transfer as an Emerging Technique for Monitoring Protein-Protein Interactions in Vivo: BRET vs. FRET | p. 529 |
BRET[superscript 2]: Efficient Energy Transfer from Renilla Luciferase to GFP[superscript 2] to Measure Protein-Protein Interactions and Intracellular Signaling Events in Live Cells | p. 539 |
High-Throughput Screening of Arabidopsis Mutants with Deregulated Stress-Responsive Luciferase Gene Expression Using a CCD Camera | p. 557 |
NorthStar HTS Workstation: A CCD-Based Integrated Platform for High-Throughput Screening | p. 565 |
Versatile, Fast Multilabel-Biochip-Reader Using a CCD Camera | p. 575 |
Index | p. 581 |
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