Malaria Methods and Protocols

By: Denise L. Doolan (Editor)

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Hardcover | 28 June 2002

At a Glance

Hardcover
648 Pages

Dimensions(cm)
25.4 x 17.8 x 3.51

New Edition

Published: 19th September 2012

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The Plasmodium spp. parasite was identified as the causative agent of malaria in 1880, and the mosquito was identified as the vector in 1897. Despite subsequent efforts focused on the epidemiology, cell biology, immunology, molecular biology, and clinical manifestations of malaria and the Plasmodium parasite, there is still no licensed vaccine for the prevention of malaria. Physical barriers (bed nets, window screens) and chemical prevention methods (insecticides and mosquito repellents) intended to interfere with the transmission of the disease are not highly effective, and the profile of resistance of the parasite to chemoprophylactic and chemotherapeutic agents is increasing. The dawn of the new millennium has seen a resurgence of interest in the disease by government and philanthropic organizations, but we are still faced with compl- ities of the parasite, the host, and the vector, and the interactions among them. Malaria Methods and Protocols offers a comprehensive collection of protocols describing conventional and state-of-the-art techniques for the study of malaria, as well as associated theory and potential problems, written by experts in the field. The major themes reflected here include assessing the risk of infection and severity of disease, laboratory models, diagnosis and typing, molecular biology techniques, immunological techniques, cell biology techniques, and field applications.

Industry Reviews

"In this book, internationally respected scientists and clinicians describe in step-by-step detail their most useful conventional and cutting-edge techniques for the study of malaria." - Clinical Laboratory International

"This is an outstanding collection of protocols written by over eighty experts in the field. The geographical spread of these contributors is impressive, encompassing writers from thirteen countries on six continents. There is good stylistic continuity throughout the book, despite the diversity of the authors...there is admirable cross-referencing between chapters." -Molecular Biotechnology

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You Can Find This Book In

Non-Fiction Medicine Clinical & Internal Medicine Diseases & Disorders Infectious & Contagious Diseases Medicine in General Medical Equipment & Techniques Medical Research Science Science in General Other Branches of Medicine Pathology

Medical Parasitology Environmental Medicine Tropical Medicine Biology, Life Sciences Microbiology excluding Medical Immunology Medical Laboratory Testing & Techniques

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Paperback

Published: 13th October 2011

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Vector Incrimination and Entomological Inoculation Rates
Epidemiological Measures of Risk of Malaria
Maintenance of the Plasmodium berghei Life Cycle
Mouse Models for Pre-Erythrocytic-Stage Malaria
Mouse Models for Erythrocytic-Stage Malaria
Nonhuman Primate Models: I. Nonhuman Primate Host-Parasite Combinations
Nonhuman Primate Models: II. Infection of Saimiri and Aotus Monkeys with Plasmodium vivax
Vector Analysis
Genotyping of Plasmodium spp.: Nested PCR
Genotyping of Plasmodium falciparum: PCR-RFLP Analysis
Microsatellite Analysis in Plasmodium falciparum
Quantitation of Liver-Stage Parasites by Automated TaqMan“ Real-Time PCR
Quantitation of Liver-Stage Parasites by Competitive RT-PCRMc
Extraction and Purification of Plasmodium Total RNA
Extraction and Purification of Plasmodium Parasite DNA
Southern Blotting of Parasite DNA
SDS-PAGE and Western Blotting of Plasmodium falciparum Proteins
Nested PCR Analysis of Plasmodium ParasitesSnounou, Georges
RFLP Analysis
Analysis of Gene Expression by RT-PCR
In Situ Detection of RNA in Blood- and Mosquito-Stage Malaria Parasites
Purification of Chromosomes from Plasmodium falciparum
Construction of Genomic Libraries from the DNA of PlasmodiumSpecies
Construction of a Gene Library with Mung Bean Nuclease-Treated Genomic DNA
Construction of Plasmodium falciparum” cDNA Libraries
Production of Stage-Specific Plasmodium falciparum cDNA Libraries Using Subtractive Hybridization
Construction and Screening of YAC Libraries
Episomal Transformation of Plasmodium berghei
Gene Targeting in Plasmodium bergheiThathy
Peptide Vaccination
DNA Vaccination
Assessing Antigen-Specific CD8+ and CD4+ T-Cell Responses in Mice After Immunization with Recombinant Viruses
Assessing CD4+ Helper T-Lymphocyte Responses by Lymphoproliferation
Limiting Dilution Analysis of Antigen-Specific CD4+ T-Cell Responses in Mice
Cell Trafficking: Malaria Blood-Stage Parasite-SpecificHirunpetcharat
Assessing Antigen-Specific Proliferation and Cytokine Responses Using Flow Cytometry
Cytokine Analysis by Intracellular Staining
Assessment of Antigen-Specific CTLand CD8+-Dependent IFN-³ Responses in Mice
Assessing Antigen-Specific CD8+ CTL Responses in Humans
Human Antibody Subclass ELISA
Systemic Nitric Oxide Production in Human Malaria: I. Analysis of NO Metabolites in Biological Fluids
Table of Contents provided by Publisher. All Rights Reserved.

Malaria Methods and Protocols

At a Glance

New Edition

Hardcover

Industry Reviews

Shipping

How to return your order

Defective items

You Can Find This Book In

Other Editions and Formats

Paperback

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