Metagenomics

Preface... Table of Contents... Contributing Authors... 1. Construction of Small-Insert and Large-Insert Metagenomic Libraries Carola Simon and Rolf Daniel 2. Extraction of Total DNA and RNA from Marine Filter Samples and Generation of a Universal cDNA as Universal Template for Marker Gene Studies Dominik Schneider, Franziska Wemheuer, Birgit Pfeiffer, and Bernd Wemheuer 3. Construction and Screening Marine Metagenomic Large Insert Libraries Nancy Weiland-Brauer, Daniela Langfeldt, and Ruth A. Schmitz 4. Constructing and Screening a Metagenomic Library of a Cold and Alkaline Extreme Environment Mikkel A. Glaring, Jan K. Vester, and Peter Stougaard 5. DNA-, RNA-, and Protein-Based Stable-Isotope Probing for High-Throughput Biomarker Analysis of Active Microorganisms Eleanor Jameson, Martin Taubert, Sara Coyotzi, Yin Chen, Ozge Eyice, Hendrik Schafer, J. Colin Murrell, Josh D. Neufeld, and Marc G. Dumont 6. Assessing Bacterial and Fungal Diversity in the Plant's Endosphere Bernd Wemheuer and Franziska Wemheuer 7. Shotgun Metagenomic Sequencing analysis of Soft-Rot Enterobacteriaceae in Polymicrobial Communities James Doonan, Sandra Denman, James E. McDonald, and Peter M. Golyshin 8. Cloning and Expression of Metagenomic DNA in Streptomyces lividans and its Subsequent Fermentation for Optimized Production Yuriy Rebets, Jan Kormanec, Andriy Lutzhetskyy, Kristel Bernaerts, and Jozef Anne 9. Degradation Network Reconstruction Guided by Metagenomic Data Rafael Bargiela and Manuel Ferrer 10. Novel Tools for the Functional Expression of Metagenomics DNA Nadine Katzke, Andreas Knapp, Anita Loeschcke, Thomas Drepper, and Karl-Erick Jaeger 11. A Microtiter Plate-Based Assay to Screen for Active and Stereoselective Hydrolytic Enzymes in Enzyme Libraries Dominique Bottcher, Patrick Zagel, Marlen Schmidt, and Uwe T. Bornscheuer 12. Screening for Cellulase Encoding Clones in Metagenomics Libraries Nele Ilmberger and Wolfgang R. Streit 13. Liquid Phase Multiplex High-Throughput Screening of Metagenomic Libraries using p-Nitrophenyl-Linked Substrates for Accessory Lignocellulosic Enzymes Mariette Smart, Robert J. Huddy, Don A. Cowan, and Marla Trindade 14. Screening Glycosyltransferases for Polyphenol Modifications Nele Ilmberger and Ulrich Rabausch 15. Methods for the Isolation of Genes Encoding Novel PHA Matabolism Enzymes from Complex Microbial CommunitiesJiujun Cheng, Ricardo Nordeste, Maria A. Trainer, and Trevor C. Charles 16. Function-Based Metagenomic Library Screening and Heterologous Expression Strategy for Genes Encoding Phosphatase Activity Genis A. Castillo Villamizar, Heiko Nacke, and Rolf Daniel 17. Activity-Based Screening of Metagenomics Libraries for Hydrogenase Enzymes Nicole Adam and Mirjam Perner 18. Screening for N-AHSL-Based-Signaling Interfering Enzymes Stephane Uroz and Phil M. Oger 19. Mining Microbial Signals for Enhanced Biodiscovery of Secondary Metabolites F. Jerry Reen, Jose A. Gutierrez-Barranquero, and Fergal O'Gara