Contributors | p. vii |
Abbreviations | p. viii |
Preface | p. x |
Instrumentation for Genomics and Proteomics | p. 1 |
Introduction | p. 1 |
History of the microplate | p. 1 |
Microplate-handling instrumentation | p. 2 |
Applications in genomics | p. 8 |
Applications in proteomics | p. 16 |
Conclusion | p. 18 |
Arraying of PCR products onto nylon membranes | p. 20 |
Processing colony arrays | p. 21 |
Expression Microarrays | p. 23 |
Introduction | p. 23 |
Microarrays in overview | p. 23 |
Array fabrication | p. 26 |
Preparation of labelled probes | p. 29 |
Microarray hybridization | p. 29 |
Generation and analysis of expression data | p. 30 |
The need for databases | p. 33 |
Validation of gene expression changes | p. 33 |
Summary and future prospects | p. 34 |
PCR amplification of cDNAs | p. 37 |
PCR product purification by precipitation | p. 38 |
Post-processing of glass slides | p. 39 |
Fluorescent probe labelling | p. 40 |
Pre-hybridization | p. 42 |
Microarray hybridization and washing | p. 43 |
Tissue Microarrays: A High-Throughput Technology for Translational Research | p. 45 |
Introduction | p. 45 |
Tissue microarray construction | p. 46 |
Staining of tissue microarrays | p. 49 |
Tissue microarray data and image management | p. 51 |
Applications in translational research | p. 54 |
Validation of tissue microarrays | p. 57 |
Future developments | p. 58 |
Protein Chips and Microarrays | p. 61 |
Introduction | p. 61 |
Microarray technology | p. 64 |
Versatility of protein microchips | p. 77 |
Concluding remarks | p. 85 |
Single Nucleotide Polymorphism Genotyping Using Microarrays | p. 89 |
Introduction | p. 89 |
DNA microarrays for sequence analysis | p. 89 |
Arrays for resequencing and SNP-scoring | p. 92 |
Summary and future directions | p. 101 |
Multiplexed allele-specific extension coupled with tag-arrays | p. 109 |
Microplate-Based mRNA Analysis | p. 115 |
Introduction | p. 115 |
Microplate-based RNA purification | p. 116 |
Microplate-based PCR | p. 119 |
Microplate-based non-RT-PCR assays | p. 123 |
Application of mRNA analysis to drug discovery project | p. 124 |
Application of mRNA analysis to clinical diagnostics | p. 125 |
A Generalized 96-Well ELISA-Based Assay for Quantitative and Qualitative Monitoring of Cellular Events | p. 131 |
Introduction | p. 131 |
The 96-well in situ ELISA assay | p. 133 |
Technical considerations | p. 134 |
Suggested statistical treatment | p. 137 |
Additional applications of the assay | p. 140 |
Conclusion | p. 140 |
The 96-well in situ ELISA assay | p. 142 |
Electrophoresis in Microplate Formats | |
Introduction | p. 145 |
MADGE, 96-well formats | p. 148 |
192-Well, 384-well, 768-well MADGE, ARMS and SNP typing | p. 152 |
96-Well high-resolution MADGE | p. 155 |
Temporal thermal ramp MADGE electrophoresis (Melt-MADGE) | p. 156 |
Software applications (applies to all MADGE approaches) | p. 160 |
The future | p. 162 |
384-Well MADGE | p. 165 |
Appendix | p. 171 |
Index | p. 173 |
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