Modern Clinical Molecular Techniques

PrefaceAcknowledgementContributors Section I Current Molecular Laboratory Methods 1 Specimen Collection, Handling and ProcessingLindsy Hengesbach and John Gerlach 2 DNA/RNA Isolation and QuantitationJing Wang 3 Test ValidationCindy McCloskey and S Terence Dunn 4 In Vitro Amplification Methods in Molecular DiagnosticsC. Alexander Valencia and Bradford Coffee 5 Whole Genome Sequencing in the Clinical LaboratoryTina Hambuch, Brad Sickler, Arnold Liao, Suneer Jain, and Philip D. Cotter 6 Estimation of Exon Dosage Using Real-Time Quantitative Polymerase Chain ReactionJuliana Purrazzella and Arupa Ganguly 7 Reporting Clinical Molecular Genetic Laboratory ResultsVictor Wei Zhang and Lora J. H. Bean Section II Clinical Molecular Infectious Disease Testing 8 HIV-1 Genotyping and Virtual PhenotypingFangling Xu and Jianli Dong 9 COBAS (R) Ampliprep/Taqman HIV-1 TestTavat A. Buraruk and Charles E. Stager 10 Hepatitis C Virus (HCV) Genotype Assay (LiPA)Charles E. Stager and Tavat A. Buraruk 11 COBAS (R) Ampliprep/Taqman HCV TestCharles E. Stager and Tavat A. Buraruk 12 Epstein-Barr Virus and Cytomegalovirus Viral Load Monitoring by Quantitative Real-Time Polymerase Chain ReactionSu S. Chen, Seema Hai, and Rajyalakshmi Luthra 13 Quantification of BK Viral LoadHai Wu and Jianli Dong 14 Molecular Diagnosis and Epidemiology of RabiesRodney E. Rohde and Bonny C. Mayes 15 Pyrosequencing for Bacterial IdentificationLaura Millecker and Ruth Ann Luna 16 Other Pathogens of Significant Public Health ConcernChris L. McGowin, Rodney E. Rohde, and Greg C. Whitlock Section III Clinical Molecular Oncology Testing 17 Gene Rearrangements TestingSiby Sebastian, Juan Du, and Rizwan Naeem 18 Mutation Analysis in CancerKeyur Pravinchandra Patel, John Galbincea, and Rajyalakshmi Luthra 19 Minimal Residual DiseaseWesley Greaves and Rajyalakshmi Luthra 20 Pyrosequencing in CancerBedia A. Barkoh, Rajyalakshmi Luthra, and Shalini Verma 21 Clinical Applications of Microarrays in CancerMarilyn M. Li, Ankita Patel, and Xiaofeng Hu 22 Basic Theories and Clinical Applications of Molecular Flow CytometryHua Kang Wu, Hongli Chen, and Peter Hu Section IV Clinical Molecular Pre/Postnatal Testing 23 Molecular Prenatal Diagnostic TestingJoanna Wiszniewska and Christine M. Eng 24 PCR Based Diagnosis of Fragile X Syndrome Daniel J. Dykas and Allen E. Bale Section V Human Identity Testing 25 Microsatellite Testing Short Tandem Repeat Profile GenerationRobin DeVille Guidry and Laura Gahn 26 ParentageLaura Gahn and Robin DeVille Guidry Section VI Clinical Molecular Training 27 Diagnostic Molecular Training Peter Hu, Patrick A. Lennon, and Madhuri Hegde 28 Molecular Diagnostics Point of Care Testing TrainingChang W. Lee, and Peter Hu