Molecular Beacons

Signalling Nucleic Acid Probes, Methods, and Protocols

By: Andreas Marx (Editor), Oliver Seitz (Editor)

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Hardcover | 2 May 2012

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280 Pages

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23.39 x 15.6 x 1.75

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Significant DNA sequence data has been made available by several important genome projects. The allocation of functions to a mere string of the four letters in the code of life plays a major if not predominant role of ongoing and future research. Guided by experts in the field, Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols, provides easy-to-follow experiments, commences with an introduction to the basic principles of fluorescence, and shows a diverse set of instructive examples, from probe design to applications in clinical settings, molecular beacon technology, the identification of transgenic mice, along with the potential of realtime PCR. While originally being used for fluorogenic nucleic acid probes with a stem-loop structure, molecular beacon now refers to a whole family of fluorescence-up probes. Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols summarizes all the latest findings and explores powerful techniques designed for geneticist, biotechnologists, molecular and cellular biologists, researchers and developers.

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Preface	p. v
Contributors	p. xi
Introduction
Basic Principles of Fluorescence and Energy Transfer	p. 3
Applications
Multiplex Detection of Mutations	p. 23
Detection of Salmonella Species in Foodstuffs	p. 33
Identification of Homozygous Transgenic Mice by Genomic Real-Time PCR	p. 45
Quantitative Analysis of Gene Expression Relative to 18S rRNA in Carcinoma Samples Using the LightCycler Instrument and a SYBR GreenI-based Assay: Determining FAT10 mRNA Levels in Hepatocellular Carcinoma	p. 59
Absolute Quantitation of Feline Leukemia Virus Proviral DNA and Viral RNA Loads by TaqMan Real-Time PCR and RT-PCR	p. 73
High-Throughput Real-Time PCR	p. 89
Design and Use of Scorpions Fluorescent Signalling Molecules	p. 99
Hybridization Probe Pairs and Single-Labeled Probes: an Alternative Approach for Genotyping and Quantification	p. 117
New Technologies
EasyBeacons for the Detection of Methylation Status of Single CpG Duplets	p. 137
Quenched Autoligation Probes	p. 161
HyBeacon Probes for Rapid DNA Sequence Detection and Allele Discrimination	p. 171
FIT-Probes in Real-Time PCR	p. 187
SNP Genotyping by Unlabeled Probe Melting Analysis	p. 199
Non-Nucleic Acid Targets
Molecular Beacons for Protein-DNA Interaction Studies	p. 209
DNA Polymerase Profiling	p. 225
Modular Reporter Hairpin Ribozymes for Analyzing Molecular Interactions	p. 237
Screening of Molecular Interactions Using Reporter Hammerhead Ribozymes	p. 251
Index	p. 265
Table of Contents provided by Ingram. All Rights Reserved.

Molecular Beacons

Signalling Nucleic Acid Probes, Methods, and Protocols

At a Glance

Hardcover

Shipping

How to return your order

Defective items

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Paperback

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