mTOR

1. Mammalian Target of Rapamycin: A Signaling Kinase for every Aspect of Cellular Life Thomas Weichhart 2. Biochemical and Pharmacological Inhibition of mTOR by Rapamycin and an ATP-Competitive mTOR Inhibitor Ker Yu and Lourdes Toral-Barza 3. Evaluation of the Nutrient-Sensing mTOR Pathway Sungki Hong, Aristotle M. Mannan and Ken Inoki 4. mTOR Activity Under Hypoxia Douangsone D. Vadysirisack and Leif W. Ellisen 5. Isolation of the mTOR Complexes by Affinity Purification Tattym Shaikenov, Olga Bulgakava, Rakhment I. Bersimbaev and Dos D. Sarbassov 6. An in vitro Assay for the Kinase Activity of mTOR Complex 2 Jingxiang Huang 7. Overexpression or Downregulation of mTOR in Mammalian Cells Mahmoud Khalil and Ivan Gout 8. Dectection of Cytoplasmic and Nuclear Functions of mTOR by Fractionation Margit Rosner and Markus Hengstschlager 9. Evaluation of Rapamycin-Induced Cell Death Lorenzo Galluzzi, Eugenia Morselli, Oliver Kepp, Ilio Vitale, Amena Ben Younes, Maria Chiara Maiuri and Guido Kroemer 10. Evaluation of mTOR-Regulated mRNA Translation Valentina Iadevaia, Xuemin Wang, Zhong Yao, Leonard J. Foster and Christopher G. Proud 11. A Genome-wide RNAi Screen for Polypeptides that Alter rpS6 Phosphorylation Angela Papageorgiou and Joseph Avruch 12. Immunohistochemical Analysis of mTOR Activity in Tissues Jinhee Kim, Nancy Otto, Claudio J. Conti, Irma B. Gimenz-Conti and Cheryl L. Walker 13. Assessing Cell Size and Cell Cycle Regulation in Cells with Altered TOR Activity Megan Cully and Julian Downward 14. Quantitative Visualization of Authophagy Induction by mTOR Inhibitors Beat Nyfeler, Philip Bergman, Christopher J. Wilson and Leon O. Murphy 15. The in vivo Evaluation of Active-Site TOR Inhibitors (asTORi) in Models of BCR-ABL+Leukemia Matthew R. Janes and David A. Fruman 16. Inducible raptor and rictor Kncokout Mouse Embryonic Fibroblasts Nadine Cybulski, Vittoria Zinzalla, and Michael N. Hall 17. Expanding Human T Regulatory Cells with the mTOR- Inbibitor Rapamycin Manuela Battaglia, Angela Stabilini and Eleonora Tresoldi 18. Rapamycin Induced Enhancement of Vaccine Efficacy in Mice Chinnaswamy Jagannath and Pearl Bakhru 19. Utilizing a Retroviral RNAi System to Investigate in vivo mTOR Functions in T Cells Koichi Araki and Bogumila T. Konieczny 20. Exploring Functional in Vivo Consequences of the Selective Genetic Ablation of mTOR Signaling in T Helper Lymphocytes Greg M. Delgoffe and Jonathan D. Powell 21. Evaluating the Therapeutic Potential of mTOR Inhibitors Using Mouse Genetics Huawei Li, Jennifer L. Cotton, David A. Guertin 22. Inhibition of PI13K-Akt-mTOR Signaling in Glioblastoma by mTORC1/2 Inhibitors Qi-Wen Fan and Williams A. Weiss 23. Assessing the Functions of mTOR in Human Embryonic Stem Cells Jiaxi Zhou, Dong Li, and Fei Wang 24. Video-EEG Monitoring Methods for Characterizing Rodent Models of Tuberous Sclerosis and Epilepsy Nicholas R. Rensing, Dongjun Guo, and Michael Wong 25. A Genetic Model to Dissect the Role of Tsc-mTORC1 in Neuronal Cultures Duyu Nie and Mustufa Sahin 26. Tissue-Specific Ablation of Tsc1 in Pancreatic Beta-Cells Hiroyuki Mori and Kun-Liang Guan 27. A Mouse Model of Diet- Induced Obesity and Insulin REsistance Chao-Yung Want and James K. Liao 28. Rapamycin as Immunosuppressant in Murine Transplantation Model Louis-Marie Charbonnier and Alain Le Moine 29. Development of ATP-Competitive mTOR Inhibitors Qingsong Liu, Seong A. Kang, Carson C. Thoreen, Wooyoung Hur, Jinhua Wang, Jae Won Chang, Andrew Markhard, Jianming Zhang, Taebo Sim, David M. Sabatini and Nathanael S. Gray