Multiple Myeloma

Preface...Table of Contents...Contributing Authors...1. ELDA qASO-PCR for High Sensitivity Detection of Tumor Cells in Bone Marrow and Peripheral BloodStefanie Huhn2. EuroFlow-Based Next-Generation Flow Cytometry for Detection of Circulating Tumor Cells and Minimal Residual Disease in Multiple MyelomaLeire Burgos Rodriguez and Bruno Paiva3. Cytoplasmic Immunoglobulin vs DNA Analysis by Flow CytometryDaisy Alapat4. Deep Profiling of the Immune System of Multiple Myeloma Patients by Cytometry by Time of Flight (CyTOF)Tina Smets, Frederik Stevenaert, Homer Adams III, and Greet Vanhoof5. Fluorescence In Situ Hybridization (FISH) in Multiple MyelomaErming Tian6. Whole Exome Sequencing in Multiple Myeloma to Identify Somatic Single Nucleotide Variants and Key Translocations Involving Immunoglobulin Loci and MYCBrian A. Walker7. RNA-Sequencing from Low-Input Material in Multiple Myeloma for Application in Clinical RoutineAnja Seckinger, Tomi Bahr-Ivacevic, Vladimir Benes, and Dirk Hose8. Protocol for M3P: A Comprehensive and Clinical Oriented Targeted Sequencing Panel for Routine Molecular Analysis in Multiple MyelomaSantiago Barrio, Matteo Da Via, Laura Bruins, Thorsten Stuhmer, Torsten Steinbrunn, Max Bittrich, Hermann Einsele, Alexander Keith stewart, Esteban Braggio, and Klaus Martin Kortum9. Analysis of Circulating Tumor DNASridurga Mithraprabhu, and Andrew Spencer10. Detection of Cross-Sample Contamination in Multiple Myeloma Samples and Sequencing DataOwen W. Stephens, Tobias Meissner, and Niels Weinhold11. Analysis of Global Gene Expression ProfilesAlboukadel Kassambara and Jerome Moreaux12. Genome Wide Mapping of Methylated and Hydroxyl-Methylated Cytosines using a Modified HpaII Tiny Fragment Enrichment by Ligation Mediated PCR Tagged Sequencing ProtocolSanchari Battachariyya, Ruslana Tytarenko, Christoph Heuck, John Greally, and Amit Verma13. A Rapid and Robust Protocol for Reduced Representation Bisulfite Sequencing in Multiple MyelomaSamrat Roy Choudhury and Brian A. Walker14. Microfluidic Production and Application of Lipid Nanoparticles for Nucleic Acid TransfectionAnitha Thomas, Shyam Garg, Rebecca DeSousa, Eric Ouellet, Grace Tharmarajah, Dave Reichert, Mina Ordobadi, Shell Ip, Samuel Clarke, and Euan C. Ramsay15. Microfluidic Assembly of Liposomes with Tunable Size and Co-Loading CapabilitiesJessica R. Hoffman, Ennio Tasciotti, and Roberto Molinaro