1. Whole Genome Sequencing for Comparative Genomics and De Novo Genome Assembly Andrej Benjak, Claudia Sala, and Ruben C. Hartkoorn 2. Whole Transcriptome Sequencing for High-resolution Transcriptomic Analysis in Mycobacterium tuberculosis Andrej Benjak, Claudia Sala, and Ruben C. Hartkoorn 3. RNA Sequencing for Transcript 5'-end Mapping in Mycobacteria Scarlet S. Shell, Michael R. Chase, Thomas R. Ioerger, and Sarah M. Fortune 4. Fractionation and Analysis of Mycobacterial Proteins Megan C. Lucas, Lisa M. Wolfe, Rachel M. Hazenfield, Jade Kurihara, Nicole A. Kruh-Garcia, John Belisle, and Karen M. Dobos 5. Lipid and Lipoarabinomannan Isolation and Characterization Marie-Antoinette Laneelle, Jerome Nigou, and Mamadou Daffe 6. Metabolomics of Mycobacterium tuberculosis Madhumitha Nandakumar, Gareth A. Prosser, Luis Pedro S. de Carvalho, and Kyu Rhee 7. Electroporation of Mycobacteria Renan Goude, David M. Roberts, and Tanya Parish 8. Targeted Gene Knockout and Essentiality Testing by Homologous Recombination Krishnamoorthy Gopinath, Digby F. Warner, and Valerie Mizrahi 9. Construction of Conditional Knockdown Mutants in Mycobacteria Dirk Schnappinger, Kathryn M. O'Brien, and Sabine Ehrt 10. Mycobacterial Recombineering Kenan C. Murphy, Kadamba Papavinasasundaram, and Christopher M. Sassetti 11. In vitro Models that Utilize Hypoxia to Induce Non-replicating Persistence in Mycobacteria Charles D. Sohaskey and Martin I. Voskuil 12. Genetic Dissection of Mycobacterial Biofilms Anil K Ojha, William R. Jacobs Jr., and Graham F. Hatfull 13. Measuring Efflux and Permeability in Mycobacteria Liliana Rodrigues, Miguel Viveiros, and Jose A. Ainsa 14. Single-cell Analysis of Mycobacteria using Microfluidics and Time-lapse Microscopy Neeraj Dhar and Giulia Manina 15. Antimicrobial Susceptibility Testing for Mycobacterium sp. Delia Blanco-Ruano, David M. Roberts, Ruben Gonzalez-Del-Rio, Daniel Alvarez, M* Jose Rebollo, Esther Perez-Herran, and Alfonso Mendoza 16. Determination of Compound Kill Kinetics Against Mycobacterium tuberculosis Julie Early and Torey Alling 17. Microplate Alamar Blue Assay (MABA) and Low Oxygen Recovery Assay (LORA) for Mycobacterium tuberculosis Sanghyun Cho, Hyung Sup Lee, and Scott Franzblau 18. A Multi-Stress Model for High Throughput Screening Against Non-replicating Mycobacterium tuberculosis Ben Gold, Thulasi Warrier, and Carl Nathan 19. Isolation and Characterization of Compound-resistant Isolates of Mycobacterium tuberculosis Theresa O'Malley and Eduard Melief 20. Macrophage Infection Models for Mycobacterium tuberculosis Benjamin K. Johnson and Robert B. Abramovitch 21. Infection of Human Neutrophils to Study Virulence Properties of Mycobacterium tuberculosis Tobias Dallenga, Bjorn Corleis, and Ulrich E. Schaible 22. Isolation of Bead-phagosomes to Study Virulence Function of M. tuberculosis Cell Wall Lipids Anna Christina Geffken, Emmanuel C. Patin, and Ulrich E. Schaible 23. Live Imaging of Mycobacterium marinum Infection in Dictyostelium discoideum Caroline Barisch, Ana T. Lopez-Jimenez, and Thierry Soldati 24. Testing Chemical and Genetic Modulators in Mycobacterium tuberculosis Infected Cells using Phenotypic Assays Vincent Delorme, Ok-Ryul Song, Alain Baulard, and Priscille Brodin