Part I. Introduction Overview of Novel Anticancer Drug Targets John K. Buolamwini and Haregewein Assefa Part II. Kinase Inhibitor Discovery Protocols Biomarker Assays for Phosphorylated MAP Kinase: Their Utility for Measurement of MEK Inhibition Judith S. Sebolt-Leopold, Keri Van Becelaere, Kenneth Hook, and Roman Herrera Assays for Cyclin-Dependent Kinase Inhibitors Adrian M. Senderowicz and Tyler Lahusen Identifying Inhibitors of ATM and ATR Kinase Activities Jann N. Sarkaria Part III. Angiogenesis and Metastasis Protocols The Rat Aortic Ring Assay for In Vitro Study of Angiogenesis Ronald S. Go and Whyte G. Owen Real Time In Vivo Quantitation of Tumor Angiogenesis Gregory I. Frost and Per Borgstrom Use of Tumor-Activated Hepatic Stellate Cell as a Target for the Preclinical Testing of Anti-Angiogenic Drugs Against Hepatic Tumor Development Elvira Olaso and Fernando Vidal-Vanaclocha Cell Motility, Adhesion, Homing, and Migration Assays in the Studies of Tyrosine Kinases Martin Sattler, Elizabeth Quackenbush, and Ravi Salgia Inflammatory Response of Tumor-Activated Hepatic Sinusoidal Endothelium as a Target for the Screening of Metastasis Chemopreventive Drugs Lorea Mendoza and Fernando Vidal-Vanaclocha Binding Assay for Selectins Jianing Zhang and Michiko N. Fukuda Determination of Peak Serum Levels and Immune Response to the Humanized Anti-Ganglioside Antibody-Interleukin-2 Immunocytokine Jacquelyn A. Hank, Jean E. Surfus, Jacek Gan, Amy Ostendorf, Stephen D. Gillies, and Paul M. Sondel Part IV. Immunohistochemical Assays in the Clinical Setting Immunohistochemical Determination of EGFR-Tyrosine Kinase Inhibition in Clinical Samples Shazli N. Malik, Roble G. Bedolla, Manuel Hidalgo, Michael G. Brattain, and Jeffrey I. Kreisberg Immunohistochemical Assays of Farnesyltransferase Inhibition in Patient Samples Alex A. Adjei Part V. Protein Chaperoning/Degradation Protocols Assays for HSP90 and Inhibitors Wynne Aherne, Alison Maloney, Chris Prodromou, Martin G. Rowlands, Anthea Hardcastle, Katherine Boxall, Paul Clarke, Michael I. Walton, Laurence Pearl, and Paul Workman Assays for Proteasome Inhibition Peter J. Elliott, Teresa A. Soucy, Christine S. Pien, Julian Adams, and Eric S. Lightcap Part VI. Protein-Protein and Protein-DNA Interactions The Mammalian Two-Hybrid Assay for Detection of Coactivator-Nuclear Receptor Interactions Curtis M. Tyree and Kay Klausing Preparation of DNA-Protein Complexes Suitable for Spectroscopic Analysis Nouri Neamati, Manisha Murthy, and Yun-Xing Wang Part VII. Antisense Antisense Oligonucleotide Inhibitors of MDM2 Oncogene Expression Ruiwen Zhang and Hui Wang Part VIII. Genomics Pharmacogenetic Analysis of Clinically Relevant Genetic Polymorphisms Christine M. Rose, Sharon Marsh, Margaret-Mary Ameyaw, and Howard L. McLeod Gene Expression Microarrays Christopher P. Kolbert, William R. Taylor, Kelly L. Krajnik, and Dennis J. O'Kane Methods for Isolation and Genetic Analysis of Circulating Tumor DNA in Patient Plasma Tomoya Kawaguchi, Will S. Holland, and Paul H. Gumerlock Part IX. Cell Lifespan/Longevity The Use of Early Sea Urchin Embryos in Anticancer Drug Testing David Nishioka, Vanessa Marcell, Meghan Cunningham, Merium Khan, Daniel D. Von Hoff, and Elzbieta Izbicka Part X. In Vivo Imaging PET Screening of Anticancer Drugs: A Faster Route to Drug/Target Evaluations In Vivo Anna Fredriksson and Sharon Stone-Elander Part XI. Miscellaneous Protocols Assays for In Vitro and In Vivo Synergy Beverly A. Teicher Flow Cytometric Methods for Detection and Quantification of Apoptosis David P. Steensma, Michael Timm, and Thomas E. Witzig Assays for Neoplastic Cell Enrichment in Bone Marrow Samples Rafael Fonseca and Gregory J. Ahmann Index
