Placenta and Trophoblast

Methods and Protocols, Volume I

By: Michael J. Soares (Editor), Joan S. Hunt (Editor)

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Hardcover | 1 November 2005

At a Glance

Hardcover
492 Pages

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23.39 x 15.6 x 2.69

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A collection of cutting-edge laboratory techniques for the study of trophoblast and placental biology. The techniques presented range from experimental animal models, to animal and human placental organ and cell culture systems, to morphological, biochemical, and molecular strategies for assessing trophoblast/placental growth, differentiation and function. Volume 1 provides readily reproducible protocols for studying embryo-uterine implantation, trophoblast cell development, and the organization and molecular characterization of the placenta. Highlights include strategies for the isolation and culture of trophoblast cells from primates, ruminants, and rodents, and precise guidance to the molecular and cellular analysis of the placental phenotype. A companion second volume concentrates on methods for investigating placental function.

Industry Reviews

"...provides step-by-step instructions for conducting research in mammalian embryology." -Weighted Numerical Score: 93 - 4 Stars!-Doody's Health Science Book Review Journal

"...an important contribution to the literature...recommend it for all those interested in learning more about maternal-fetal interactions during pregnancy." -Weighted Numerical Score: 93 - 4 Stars!-Doody's Health Science Book Review Journal

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You Can Find This Book In

Non-Fiction Medicine Clinical & Internal Medicine Paediatric Medicine Neonatal Medicine Medicine in General Medical Equipment & Techniques Medical Research Pre-Clinical Medicine & Basic Sciences Human Reproduction, Growth & Development Gynaecology & Obstetrics Materno-Fetal Medicine

Physiology Science Biology, Life Sciences Molecular Biology Nursing & Ancillary Services Nursing

Preface	p. v
Contributors	p. xi
Companion Table of Contents for Volume I	p. xvii
Companion CD-ROM	p. xxi
Introduction
Placenta and Trophoblast: Methods and Protocols: Overview II	p. 3
Analysis of Trophoblast Invasion
In Vivo Analysis of Trophoblast Cell Invasion in the Human	p. 11
In Vitro Analysis of Trophoblast Invasion	p. 45
An In Vitro Model of Trophoblast Invasion of Spiral Arteries	p. 59
Analysis of Uteroplacental Immune Cells and Their Functions
In Vivo Models for Studying Homing and Function of Murine Uterine Natural Killer Cells	p. 77
Immune and Trophoblast Cells at the Rhesus Monkey Maternal-Fetal Interface	p. 93
Methods for Isolation of Cells from the Human Fetal-Maternal Interface	p. 109
In Vitro Models for Studying Human Uterine and Placental Macrophages	p. 123
Macrophage-Trophoblast Interactions	p. 149
Methods for Evaluating Histocompatibility Antigen Gene Expression in the Baboon	p. 165
Analysis of the Soluble Isoforms of HLA-G: mRNAs and Proteins	p. 181
Analysis of Placental Function: Transport and Endocrinology
In Vivo Techniques for Studying Fetoplacental Nutrient Uptake, Metabolism, and Transport	p. 207
In Vitro Models for Studying Trophoblast Transcellular Transport	p. 225
In Vitro Methods for Studying Human Placental Amino Acid Transport: Placental Plasma Membrane Vesicles	p. 241
In Vitro Methods for Studying Human Placental Amino Acid Transport: Placental Villous Fragments	p. 253
Methods for Investigating Placental Fatty Acid Transport	p. 265
Heterologous Expression Systems for Studying Placental Transporters	p. 285
Analysis of Trophoblast Giant Cell Steroidogenesis in Primary Cultures	p. 301
Establishment of an ELISA for the Detection of Native Bovine Pregnancy-Associated Glycoproteins Secreted by Trophoblast Binucleate Cells	p. 321
Alkaline Phosphatase Fusion Proteins as Tags for Identifying Targets for Placental Ligands	p. 331
Bacterial Expression of Prolactin Family Proteins	p. 341
Analysis of Placental Regulation of Hematopoiesis	p. 355
Methods for Studying Interferon Tau Stimulated Genes	p. 367
Analysis of Placenta Adaptation to Disease
Reduced Uterine Perfusion Pressure (RUPP) Model for Studying Cardiovascular-Renal Dysfunction in Response to Placental Ischemia	p. 383
In Vivo Rat Model of Preeclampsia	p. 393
A Novel Mouse Model for Preeclampsia by Transferring Activated Th1 Cells into Normal Pregnant Mice	p. 401
Working with Oxygen and Oxidative Stress In Vitro	p. 413
Hypobaric Hypoxia as a Tool to Study Pregnancy-Dependent Responses at the Maternal-Fetal Interface	p. 427
Infection with Listeria monocytogenes as a Probe for Placental Immunological Function	p. 435
Index	p. 443
Table of Contents provided by Ingram. All Rights Reserved.

Placenta and Trophoblast

Methods and Protocols, Volume I

At a Glance

Hardcover

Industry Reviews

Shipping

How to return your order

Defective items

You Can Find This Book In

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