Section 1 General Introduction 1. Plant virus interactions --Peter Palukaitis, John Carr and James Schoelz (EJ) 2. Role of capsid proteins -- John Bol (EJ) 3. Role of plant virus movement proteins -- Michael Taliansky, Lesley Torrance, Natalia O. Kalinina (EJ) 4. Multiple roles of viral replication proteins in plant RNA virus replication-- Peter Nagy and Judith Pogany (PN) 5. Role of silencing suppressor proteins --Jozsef Burgyan (EJ) 6. Role of vector transmission proteins -- Veronique Ziegler Graff, Veronique Brault (EJ) Section 2 Viral nucleic acid sequence/function analysis 7. In vivo analysis of Viral RNA translation- William Staplin, Allen Miller (PN) 8. In vitro analysis of translational enhancer --Aurelie Rakotondrafara, Allen Miller (YH) 9. Identification of plant virus IRES -- Sek-Man Wong, Dora Chin-Yen Koh, DingXiang Liu (YH) 10. Analysis of geminivirus DNA replication by 2-D gel - Keith Saunders (YH) 11. Begomoviruses: Molecular cloning and identification of replication origin - Lilian H. Florentino, Anesia A. Santos, Francisco M. Zerbini, Elizabeth P. B. Fontes (YH) 12. Analysis of viroid replication - Ricardo Flores, Maria-Eugenia Gas, Diego Molina, Carmen Hernandez, and Jose-Antonio Daros (PN) 13. Biochemical Analyses of the Interactions Between Viral Polymerases and RNAs- Young-Chan Kim and C. Cheng Kao (PN) 14. In situ detection of plant viruses and virus-specific products - Andy Maule and Zoltan Havelda (PN) 15. Detection of siRNAs and miRNAs- Sakari Kauppinen, Zoltan Havelda (EJ) 16. Cloning of short interfering RNAs from virus infected plants - Thien X. Ho, Rachel Rusholme, Tamas Dalmay, and Hui Wang (YH) 17. Solution structure probing of RNA structures -- Marc R. Fabian and K. Andrew White (PN) 18. RNA encapsidation assay - Padmanaban Annamalai and A. L. N. Rao (GF) Section 3 Protein analysis and investigation of protein function 19. Surface plasmon resonance analysis of interactions between replicase proteins of tomato bushy stunt virus- KS. Rajendran and Peter Nagy (GF) 20. Biochemical approaches for characterizing RNA-protein complexes in preparation for high resolution structure analysis- Raul C. Gomila and Lee Gehrke (PN) 21. Probing interactions between plant virus movement proteins and nucleic acids - Tzvi Tzfira, Vitaly Citovski (PN) 22. Movement profiles, a tool for quantitative analysis of cell-to-cell movement of plant viral movement proteins - Kateryna Trutnyeva, Pia Ruggenthaler, Elisabeth Waigmann (PN) 23. Analysis of siRNA-supressor of gene silencing interactions - Lorant Lakatos and Jozsef Burgyan (EJ) 24. Phosphorylation analysis of plant virus proteins - Kristiina M. Makinen and Konstantin I. Ivanov (PN) 25. Analysis of interactions between viral replicase proteins and plant intracellular membranes- Helene Sanfacon and Guangzhi Zhang (PN) 26. Membrane and protein dynamics in virus-infected plant cells - Michael Goodin, Romit Chakrabarty, Sharon Yelton (PN) 27. Site-directed mutagenesis of whole viral genomes - Li liu, George Lomonossoff (YH) 28. Viral protein-nucleic acid interaction: South(North)-Western blot - Huanting Liu (YH) 29. Protein-protein interactions: yeast two hybrid system - Deyin Guo, Minna-Liisa Rajamaki, and Jari Valkonen (YH) 30. NMR analysis of viral protein structures - Andrew J. Dingley, Inken Lorenzen and Joachim Grotzinger (YH) 31. Localization of viral protein in plant cells: protein tagging --Sophie Haupt, Lesley Torrance (GF) Section 4 Microscopy/GFP/Protein tagging/infections clones and other such tools 32. Construction of infectious clones for RNA viruses - TMV ~ Sean Chapman (YH) 33. Construction of infectious clones for RNA viruses -TCV ~ Eugene Ryabov (YH) 34. Construction of infectious clones for DNA viruses -Mastrevirus -Margaret Boulton (EJ) 35. Construction of infectious clones of double-stranded DNA viruses of plants using Citrus Yellow Mosaic Virus as an example- Qi Huang, John S. Hartung (EJ) 36. Insertion of Introns -- A Strategy to Facilitate Assembly of Infectious Full Length Clones-Elisabeth Johansen and Ole Sogaard Lund (GF) 37. Analysis of cell-to-cell and long-distance movement of Apple latent spherical virus in infected plants using green, cyan, and yellow fluorescent proteins -- Tsubasa Takahashi, Nobuyuki Yoshikawa (GF) 38. Agroinoculation: A simple procedure for systemic infection of plants with virus -- Zarir Vaghchhipawala, Kirankumar Mysore (GF) 39. Geminvirus biolistic inoculation and molecular diagnosis -- Anesia A. Santos, Lilian H. Florentino, Acassia M. B. Leal, Elizabeth P. B. Fontes (YH) Section 5 Genomics, Host factors and plant based studies 40. Expression microarrays in plant-virus interactions- Gruden K., Pompe-Novak M., Baebler S., Krecic-Stres H., Toplak N., Hren M., Kogov sek, P., Gow L., Foster G., Boonham N., Ravnikar M. (GF) 41. Genome-wide screens for identification of host factors in viral replication- Tadas Panavas, Elena Serviene, Judit Pogany and Peter D. Nagy (PN) 42. Phosphorylation of Movement Proteins by the Plasmodesmal-Associated Protein Kinase- Jung Younlee (GF) 43. Virus induced gene silencing as a tool to identify host genes affecting viral pathogenicity - Xiaohong Zhu and Dinesh-Kumar, S.P (GF) 44. Yeast Two-hybrid assay to identify host-virus interactions- Stuart A. MacFarlane and Joachim F. Uhrig (EJ)
