Progress in Plant Protoplast Research

Progress in Plant Protoplast Research.- Obstacles and Perspectives in Protoplast Research.- Aspects of Protoplast Culture and Plant Regeneration.- Recent Developments in the Regeneration of Agronomically Important Crops from Protoplasts.- Selection, Experimental Manipulation and Culture of Single Protoplasts.- Uniparental, Fusant, and Chimeric Plants Regenerated from Protoplasts after Streak Plating in Agarose Gels.- Electroporation Mediated Enhancement of Plant Protoplast Division and Plant Regeneration.- Selection of Potato Cell Suspension Protoplast Subpopulation by Rhodamine 123.- Protoplast Culture of Dioscorea opposita Thunb. (D. Batatas Decne).- Isolation and Culture of Buckwheat (Faqopyrum esculentum Moench.) Callus Protoplasts.- Partial Purification of a Cellulase Used for Protoplast Isolation from Barley (Hordeum vulgare).- Plant Regeneration from Cell Suspension Protoplasts of Festuca arundinacea Schreb., Lolium perenne L. and L. multiflorum Lam..- Isolation, Culture and Regeneration of Lolium perenne and Lolium multiflorum Protoplasts.- Mature Plant Regeneration from cultivated Protoplasts of Rice (Oryza sativa L.).- The Plants Regenerated from Protoplasts of Suspension Cells of Rice (Oryza sativa L).- Regeneration of Plantlets from Protoplasts of Poa pratensis L. (Kentucky Bluegrass).- Somatic Embryogenesis and Plant Regeneration from Protoplast of Maize (Zea mays L.).- Protoplasts from Actinidia deliciosa (Kiwi): Obtention and Culture.- Colony Formation from Cell Suspension Culture Protoplasts of Kiwifruit.- Isolation and Culture of Grapevine Protoplasts (Vitis Vinifera L.).- Studies Towards a Protoplast Isolation and Culture Procedure in Sugarbeet (Beta vulgaris L.) Applicable to a Variety of Genotypes.- Improvement of Protoplast Culture in Helianthus annuus (L).- Factors Influencing Protoplasts Plating Efficiency and Direct Somatic Embryogenesis Induction in Sunflower (Helianthus annuus L.).- Comparison of Sunflower Protoplast Development in Liquid or Solidified Media.- Plant Regeneration from Protoplasts of Rudbeckia and Dimorphotheca Species (Compositae).- Optimization of Lettuce Calli Regeneration from Protoplasts.- Optimal Density and Osmolality of Protoplasts for Improved Cultural Performance.- Development of Protoplast Culture Procedures in Brassica Crops Suitable for in vitro Selection Herbicide Resistance Mutants and Cybrids.- Somatic Embryogenesis in Stationary Phase Suspension Cultures Derived from Hypocotyl Protoplasts of Brassica napus L..- Plant Regeneration from Cucumber Protoplasts.- Callus Formation from Mesophyll protoplasts of Forage Legume Astragalus huangheensis Fu et Lin.- Preliminary Studies of Lupinus Protoplasts.- Regeneration and Differentiation of Protoplasts in Grain Legumes.- Plant Regeneration from Strawberry (Fragaria ananassa) Mesophyll Protoplasts.- Isolation and Culture of Apple Mesophyll Protoplasts.- Phizogenesis as an Intermediate Step Towards Plant Regeneration of Protoolasts of Sour Cherry (Prunus cerasus L.) Rosaceae.- Isolation and Culture of Protoplasts from Totipotent Cell Cultures of Populus ciliata.- A Protoplast System for Populus which will Support Genetic Engineering Research of Tree Species.- Factors Influencing Digitalis obscura Mesophyll Protoplast Development.- Cotyledon Protoplasts from Solanum melongena L. Regeneration to Fertile.- Isolation, Culture and Regeneration of Leaf Protoplasts in the Genus Capsicum.- Isolation and Properties of Protoplasts of Theobroma cacao L..- Plantlet Regeneration from Protoplasts of Levisticum officinale Koch. and its Cytological Investigation.- Differences in Developmental Morphology of Protoplasts from Two Cell lines of which Spruce (Picea glauca).- Protoplast Development from Porphyra linearis - An Edible Marine Red Alga.- Structure and Physiology of the Protoplast Plasma Membrane.- Why do Mesophyll Protoplasts Dedifferentiate?.- Release of Phytotoxic Factors from Plant Cell Walls During Protoplast Isolation.- Role of Peroxidase in Protoplast Development: The Activity and Molecular Forms of Peroxidase in Mitogenic and Non-Mitogenic Protoplasts.- Measurement of Cytoplasmic CA2+ and H+ in Barley Aleurone Protoplasts.- The Effect of Secretion on Lipids and Their Distribution in Barley Aleurone Protoplasts.- Incidence of Growth Regulators on RNA/DNA Levels in Protoplasts as Analysed by Acridine Orange Staining and Flow Cytometry.- IAA, ABA Content and Metabolism in Maize Root Protoplasts.- Some Metabolic Peculiarities of Isolated Protoplasts.- Gene Transfer by Individual Manipulation of Plant Cells.- Potentials of Protoplast Fusion in Plant Breeding Programmes.- Use of Protoplasts in Plant Breeding: Cytoplasmic Aspects.- Protoplast Fusion between Eruca sativa and Brassica napus - Analysis of Regenerated Plants.- Fertility and Chromosome Stability in Brassica napus Resynthesised by Protoplast Fusion.- Involvement of Protoplast Fusion in Cauliflower Breeding.- Protoplast Fusion as a Method of Increasing Cytoplasmic Heterogeneity in Brassica napus.- Selection of a Universal Hybridizer in Sinapis turgida Del. and Regeneration on Plantlets from Somatic Hybrids with Brassica species.- Towards the Introduction of Cytoplasmic Male Sterility (CMS) into Brassica napus Through Protoplast Fusion.- Production of Somatic Hybrid Plants, 'Brassicnmoricandia', Through Protoplast Fusion between Moricandia arvensis and Brassica oleracea.- The Use of Haploid Protoplast Fusion to Combine Cytoplasmic Atrazine Resistance and Cytoplasmic Male Sterility in Brassica napus.- Somatic Hybridization of Diploid Potato Lines, A Tool in Potato Breeding.- Somatic Hybridization between Anther-Derived Dihaploid Lines of Solanum tuberosum L. (Potato).- Fusion of Dihaploid Solanum tuberosum Protoplasts and Trials of Hybrid Selection with Phytotoxins.- Production and Field Assessment of Somatic Hybrids between Dihaploid Solanum tuberosum and Solanum brevidens.- Fusion of Solanum tuberosum L. cv. Explorer (Pototo) and Solanum melongena L. cv. Imperial Black Beauty (Eggplant) Protoplasts and Subseguent Callus Regeneration.- Somatic Fusions of Solanum Species.- Flow Cytometric Sorting of Potato Heterokaryons with Inhibition of Cell Wall Synthesis Before Sorting.- An Improved Peg Fusion Procedure for Flow Sorting of Plant Protoplasts.- Regeneration and Evaluation of Somatic Hybrid Plants between Solanum tuberosum and Lycopersicon pimpinellifolium.- Studies of Gene Mapping in the Potato Using Somatic Hybrids of Solanum tuberosum L. + Nicotiana plumbaginifolia.- Interspecific Expression of AEC-Resistance in Somatic Hybrids of AEC-Resistant Solanum tuberosum and Nitrate-Reductase-Deficient Nicotiana plumbaginifolia.- Interspecific Protoplast Fusion for the Transfer of Atrazine Resistance from Solanum nigrum to Tomato (Lycopersicon esculentum. L.).- Somatic Hybridization between Tomato and Other Lycopersicon or Solanum Species.- Transfer of Regeneration Capacity from Lycopersicon peruvianum to L. esculentum by Protoplast Fusion.- Somatic Hybridization in Lycopersicon; Using Gamma-Irradiated Nicotiana tabacum SR-1 or Lycopersicon peruvianum.- Regeneration of Somatic Hybrid Plants between Tobacco (Nicotiana tabacum L.) and Black Nightshade (Solanum nigrum L.).- The Use of Streptomycin Resistance and Chlorophyll Deficiency for Selection of Somatic Hybrids Between Nicotiana tabacum and N. rustica.- Intra- and interspecific Gametosomatic Hybridisation Within the Genus Petunia.- The Transfer of Cytoplasmic Male Sterility (CMS) by Intraspecific Somatic Hybridisation in Petunia hybrida (Hort) VILM.- Applimation of Protonlast Fusion to an Ornamental Breeding Programme.- Formation of Carrot Cybrids Based on Metabolic Complementation by Fusion between X-Irradiated Cells and Iodoacetamide-Treated Cells.- Optimization of Conditions for Somatic Cybridization of Daucus carota L..- Isolation and Fusion of Apium graveolens (Celery) and Levisticum officinale (Lovage) Mesophyll Protoplasts.- Electrofusion of Plant and Fungal Protoplasts.- Regeneration and Analysis of Callus from Flow-Sorted Heterokaryons of Soybean (Glycine max) and G. canescens.- Interspecific Somatic Hybridization Studies in the Genus Lotus.- Interspecific Hybrid Lines of M. sativa and M. arborea by Protoplast Electrofusion.- Electrofusion and Electroporation of Sugar Beet (Beta vulgaris L.) Protoplasts.- The Production and Electrofusion of Beta cytoplasts.- Inactivation of Sugarbeet Protoplasts Using Acridin Orange, an Agent for Late Selection of Fusion Products.- Mitochondrial DNA Amplification in Somatic Hybrid Cells of Grasses.- Plant Regeneration from Electrofused Protoplasts of Chrysanthemum morifolium and Salpiglossis sinuata.- Electrofusion of Chemically-Aggregated Protoplasts Derived from Mesophyll Tissue and Habituated Cells of Catharanthus roseus.- Studies on the Tranfer of the Mitochondrial Genome Through Protoplast Fusion in Rice.- Increase of Genetic Variability by Asymmetric Cell Hybridization and Isolated Chromosome Transfer.- Cell-Mediated Gene Transfer.- Protoplast Fusion of Kanamycin Resistant, CNX- Nicotiana plumbaginifolia with Streptomycin Resistant N. tabacum (SR1) and the Effect of Irradiation of the N. tabacum Parent..- Selection for Resistance to Phoma Lingam Using Pycnospore Infections of Plants In-Vivo and Sirodesmin PL as Selective Agent In-Vitro.- Induction of Micronuclei by Amiprophos-Methyl and Their Application for Transfer of Single, Specific Chromosomes in Plant Cells.- Isolation of Microprotoplasts.- Direct Cell to Cell Transfer of Mitochondria by Microinjection.- Classification and Sorting of Plant Chromosomes Using Flow Cytometry.- Application of Flow Cytometry for the Isolation of Metaphase Chromosomes from Protoplasts of Nicotiana plumbaginifolia.- Electroporation for DNA Delivery to Plant Protoplasts.- Electromanipulation of Protoplasts.- Plant Protoplast Electropermeabilization: Role of Electrical and Biological Parameters.- The Fusogenic State of Plant Protoplasts as Related to DYE/DNA Uptake.- Genetic Manipulation of Plant Cells and Organelles with a Laser Microbeam.- Electric Field Mediated DNA Uptake and Gene Expression in Mesophyll, Suspension Culture, and Tuber Protoplasts of Wild and Cultivated Potato.- of Selectable Markers into Different Diploid Potato (Solanum tuberosum L.) Genotypes by Direct Gene Transfer.- Direct Gene Transfer in Petunia hybrida Protoplasts: Evidence for Co-Transformation.- Electroporation of Tobacco Protoplasts: A. Model System to Study Plant Gene Expression.- Infection of Tobacco Protoplasts with RNA of a Pepper Strain of Tobacco Mosaic Virus by Electroporation.- Comparison of Direct Gene Transfer Into Nicotiana Protoplasts by Electroporation and Mg2+/PEG Methods.- Quantification of Transient Expression Levels of Genes Transferred to Plant Protoplasts by Electroporation.- Gene Transfer by Electroporation in Alnus incana Protoplast.- Transformation of Rice Protoplasts by Direct DNA Uptake.- Isolation, Culture and Morphogenesis from Wheat Protoplasts, and Study of Expression of DNA Constructs by Direct Gene Transfer.- UV Inducible Transient Expression of Chimeric CHS-NPTII in Parsley Protoplasts.- Modification of Plants Using Intracellular Associations of L-Form Bacteria.- Transformation of Brassica nigra through Protoplast Cocultivation with Agrobacterium tumefaciens.- Transformation of Brassica Species via Direct Gene Transfer.- Genetic Transformation of Diploid Solanum tuberosum by Direct DNA Transfer and Mediated by Agrobacterium tumefaciens.- Optimization of Direct Gene Transfer in Tomato.- Transformation Studies in Tobacco, Moth Bean and Brassica Species.- Transposition and Regulation of the EN Elements of Maize Assayed in Transformed Tobacco.- Initiation of Cell Suspensions, Protoplast Culture, and Direct Gene Transfer to Protoplasts of Secale cereale L. (Rye).- Protoplast Culture and DNA Uptake in Echinocloa.- Transient Expression of Foreign Genes in Wheat Protoplasts.- Protoplast Culture and Transformation Studies of Triticale (x Triticosecale Wittmack).- Analysis of the Zea Mays Sucrose Synthase Promotor by Transient Gene Expression in Maize Protoplasts.- Protoplast Culture of 2n S. tuberosum as a Source of Mutant Phenotypes.- Cytoplasmic or Nuclear Control of Male Sterility in Protoolast-Derived Plants of Nicotiana sylvestris.- Selection and Characterization of Nitrate Reductase-Deficient Mutants of Nicotiana plumbaqinifolia.- Isolation of Temperature-Sensitive Auxin-Requiring Variants of Nicotiana plumbaginifolia.- The Use of Phospinothricin Resistance as a Selectable Marker in Tobacco Protoplast Transformation.- Selection for Salt/Drought Tolerance Using Isolated Protoplasts and Protoplast-Derived Calli of Colt Cherry (Prunus avium X Pseudocerasus).- Field Tests of Progeny from Protoplast Regeneration Oilseed Rape.- Studies of Lettuce Plants Regenerated from Protoplasts.- Somaclonal Variations for Indole Alkaloid Accumulation in Protoclones Derived from an Habituated Strain of Catharanthus roseus.- Somaclonal Variations of Dihydrofuroquinoline Alkaloids in Cell Lines Issued from Strains and Leaf Mesophyll of Choisya Ternata by Protoplast Cloning.- Some Aspects of Salinity Tolerance of Calli Derived from Leaf Tomato Protoplasts.- Proto-Variation in Protoplast Derived Brassica napus Plants.- Index of Organism.- Index of Authors.