Protein Bioseparation Using Ultrafiltration

Theory, Applications And New Developments

By: Raja Ghosh

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Hardcover | 12 June 2003

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188 Pages

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Ultrafiltration (UF) is a pressure-driven separation process in which membranes are used for a broad variety of applications, ranging from the processing of biological macromolecules to wastewater treatment. It has significant advantages over competing separation technologies. Food and biotechnological applications of UF account for nearly 40 percent of the current total usage. In the case of high value therapeutic protein and DNA products, the separation and purification costs can be as high as 80 percent of the total cost of production. Therefore, it makes economic sense to develop cost-effective and scaleable purification processes for such products. UF is used for protein concentration, protein desalting and protein fractionation (such as protein-protein separation). Concentration and desalting processes are technologically less demanding and have been widely used in the bioprocess industry for quite some time. Protein fractionation, on the other hand, is a challenging proposition and is definitely a more recent development. This text focuses primarily on protein fractionation.

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Dedication	p. v
Preface	p. vii
Acknowledgements	p. ix
List of Tables	p. xv
List of Figures	p. xvii
Protein Bioseparation: An Overview	p. 1
Introduction	p. 1
Proteins	p. 1
Protein products	p. 2
The requirement for protein bioseparation	p. 3
Economic aspects of protein bioseparation	p. 5
Protein bioseparation methods	p. 5
Ultrafiltration: An Overview	p. 13
Introduction	p. 13
Applications of ultrafiltration	p. 13
Advantages of ultrafiltration	p. 15
Membranes	p. 19
Introduction	p. 19
Membrane material and chemistry	p. 19
Membrane structure and morphology	p. 21
Membrane preparation	p. 23
Driving force in membrane separation processes	p. 24
Membrane characterisation	p. 26
Membrane Module and Operation	p. 33
Membrane elements and modules	p. 33
Mode of operation	p. 44
Membrane Fouling	p. 47
Introduction	p. 47
Fouling mechanisms	p. 48
Chemistry of adsorption	p. 51
Initial and long-term fouling	p. 52
Effect of concentration polarisation on fouling	p. 54
Effect of permeate flux on fouling, and the critical flux concept	p. 54
Effect of physicochemical parameters on fouling	p. 54
Permeate Flux in Ultrafiltration	p. 57
Permeate flux	p. 57
Enhancement of permeate flux	p. 63
Fouling control	p. 68
Protein Transmission Through Ultrafiltration Membranes	p. 75
Protein size	p. 75
Protein transmission	p. 75
Selectivity of Protein Fractionation in Ultrafiltration	p. 87
Selectivity	p. 87
Enhancement of selectivity	p. 89
Protein Concentration	p. 91
Introduction	p. 91
Solute retention	p. 92
Permeate flux and fouling in concentration processes	p. 94
Modules	p. 95
Mode of operation	p. 96
Applications	p. 101
Diafiltration of Protein Solutions	p. 105
Introduction	p. 105
Permeate flux and fouling in diafiltration	p. 105
Modules	p. 107
Mode of operation	p. 108
Applications	p. 108
Protein Clarification	p. 111
Introduction	p. 111
Protein transmission	p. 111
Permeate flux and fouling in protein clarification	p. 112
Particle transmission through UF membrane	p. 112
Modules	p. 113
Mode of operation	p. 114
Applications	p. 118
Protein Fractionation	p. 121
Introduction	p. 121
Challenges facing ultrafiltration based protein fractionation	p. 121
Selectivity enhancement	p. 123
Thumb rules for protein fractionation	p. 133
Application areas	p. 135
New Developments	p. 145
New membranes	p. 145
New membrane modules	p. 146
Rapid process optimisation techniques	p. 147
New modes of operation	p. 148
Glossary of Membrane Terminology	p. 155
List of Membrane Manufacturers	p. 163
Websites of Membrane Related Academic and Research Institutions	p. 165
Table of Contents provided by Rittenhouse. All Rights Reserved.

Protein Bioseparation Using Ultrafiltration

Theory, Applications And New Developments

At a Glance

Hardcover

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How to return your order

Defective items

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