Proteome Research : Mass Spectrometry

Mass Spectrometry

By: Peter James (Editor)

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Hardcover | 1 December 2000

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302 Pages

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23.39 x 15.6 x 1.75

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Recent advances in large-scale DNA sequencing technology have made it possible to sequence the entire genome of an organism. Attention is now turning to the analysis of the product of the genome, the proteome, which is the set of proteins being expressed by a cell. Two-dimensional gel electrophoresis can be used to create cellular protein maps which give a quantitative and qualitative picture of the proteome. Mass spectrometry is the method of choice for the rapid large-scale idenfification of these proteomes and their modifications. An understanding of these methods is critical for scientists in the "Post-Genome" era.

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Mass Spectrometry and the Proteome
Basics of Triple-Stage Quadrupole/Ion-Trap Mass Spectrometry: Precursor, Product and Neutral-Loss Scanning. Electrospray Ionisation and Nanospray Ionisation
The Basics of Matrix-Assisted Laser Desorption, Ionisation Time-of-Flight Mass Spectrometry and Post-Source Decay Analysis
Data-Controlled Micro-Scale Liquid Chromatography - Tandem Mass Spectrometry of Peptides and Proteins: Strategies for Improved Sensitivity, Efficiency and Effectiveness
Solid-Phase Extraction - Capillary Zone Electrophoresis-Mass Spectrometry Analysis of Low-Abundance Proteins
Protein Identification by Peptide-Mass Fingerprinting
Protein Identification by SEQUEST
Interpreting Peptide Tandem Mass-Spectrometry Fragmentation Spectra
Automated Interpretation of Peptide Tandem Mass Spectra and Homology Searching
Specific Detection and Analysis of Phosphorylated Peptides by Mass Spectrometry
Glycoproteomics: High-Throughput Sequencing of Oligosaccharide Modifications to Proteins
Proteomics Databases
Quo Vadis
Subject Index
Table of Contents provided by Blackwell. All Rights Reserved.

Proteome Research : Mass Spectrometry

Mass Spectrometry

At a Glance

Hardcover

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How to return your order

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More in Biochemistry

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