1. Platforms for Recombinant Therapeutic Glycoprotein Production Amanda Mizukami, Angelo Luis Caron, Virginia Picanco-Castro, and Kamilla Swiech 2. Uncovering Innovation Features and Emerging Technologies in Molecular Biology through Patent Analysis Cristiano Goncalves Pereira and Geciane Silveira Porto Part I: Upstream Processes 3. Production of Full-length Antibody by Pichia Pastoris Adam Nylen and Ming-Tang Chen 4. Human cells as Platform to Produce Gamma-Carboxylated Proteins Aline de Sousa Bomfim, Marcela Cristina Correa de Freitas, Dimas Tadeu Covas, and Elisa Maria de Sousa Russo 5. Production of Recombinant Factor VIII in Human Cell Lines Daianne Maciely Carvalho Fantacini, and Virginia Picanco-Castro 6. Strategies to Suspension Serum-Free Adaptation of Mammalian Cell Lines for Recombinant Glycoprotein Production Angelo Luis Caron, Rafael Tage Biaggio, and Kamilla Swiech 7. Production of Recombinant Rabies Virus Glycoprotein by Insect Cells in a Single Use Fixed-bed Bioreactor Daniella C Ventini-Monteiro, Renato M Astray, and Carlos A Pereira 8. Cell-Free Production of Protein Biologics within 24 hours Challise J. Sullivan, Erik D. Pendleton, and John Dresios 9. Demonstration-Scale High-Cell-Density Fermentation of Pichia Pastoris Wan-Cang Liu and Ping Zhu 10. Large Scale Transient Transfection of Suspension Mammalian Cells for VLP Production Laura Cervera and Amine Kamen 11. Bioreactor-Based Production of Glycoproteins in Plant Cell Suspension Cultures Tanja Holland and Johannes Felix Buyel 12. Fed-Batch CHO Cell Culture for Lab-Scale Antibody Production Yuzhou Fan, Daniel Ley, and Mikael Rordam Andersen 13. Strategies to Develop Therapeutic N- and O-Hyperglycosylated Proteins Agustina Gugliotta, Natalia Ceaglio, Marina Etcheverrigaray,and Ricardo Kratje, Marcos Oggero 14. Expression of Glycosylated Proteins in Bacterial System and Purification by Affinity Chromatography Carlos Alexandre Breyer, Marcos Antonio de Oliveira, and Adalberto Pessoa Jr Part II: Downstream Processes 15. Purification Methods for Recombinant Factor VIII Expressed in Human Liver SK-Hep Cells Granovski V, Abreu-Neto M.S, and Dimas Tadeu Covas 16. Purification Method for Recombinant hG-CSF by Affinity Chromatography Bruna Samham Archangelo and Elisa Maria de Sousa Russo 17. Microplate-Based Method for High-Throughput Screening (HTS) of Chromatographic Conditions Studies for Recombinant Protein Purification Rimenys J. Carvalho and Thayana A. Cruz 18. Purification and Auto Activation Method for Recombinant Coagulation Factor VII Vladimir Granovski, Marcela Cristina Correia de Freitas, Mario Soares de Abreu-Neto, and Dimas Tadeu Covas Part III: Formulation and Analytics 19. Preparation of Immunoliposomes by Direct Coupling of Antibodies Based on a Thioether Bond Raquel Petrilli, Josimar O. Eloy, Robert J Lee, and Renata F. V. Lopez 20. Polyester-Based Nanoparticles for the Encapsulation of Monoclonal Antibodies Flavia Sousa, Pedro Fonte, Andreia Cruz, Patrick J. Kennedy, Ines Mendes Pinto, and Bruno Sarmento 21. Polyester-Based Nanoparticles for Delivery of Therapeutic Proteins Pedro Fonte, Flavia Sousa, and Bruno Sarmento 22. Quantification of Coagulation Factor VIII by Selective Reaction Monitoring Edson Galdino do Nascimento Filho, Guilherme Pauperio Lanfredi, Mario de Abreu Soares Neto, Dimas Tadeu Covas, and Vitor Marcel Faca
