Ribosome Display and Related Technologies

Part I: Review 1. Ribosome Display: A Perspective Andreas Pluckthun Part II: Translation Extract Preparation 2. Preparation and Testing of E.coli S30 In Vitro Transcription Translation Extracts James F. Zawada Part III: Basic Ribosome Display and Related Selection Methods 3. Eukaryotic Ribosome Display Selection Using Rabbit Reticulocyte Lysate Julie A. Douthwaite 4. Stabilized Ribosome Display for In Vitro Selection Shuta Hara, Mingzhe Liu, Wei Wang, Muye Xu, Zha Li, and Yoshihiro Ito 5. Eukaryotic Ribosome Display with In Situ DNA Recovery Mingyue He, Bryan M. Edwards, Damjana Kastelic, and Michael J. Taussig 6. mRNA Display Using Covalent Coupling of mRNA to Translated Proteins Rong Wang, Steve W. Cotten, and Rihe Liu 7. SNAP Display: In Vitro Protein Evolution in Microdroplets Miriam Kaltenbach and Florian Hollfelder 8. cDNA Display: Rapid Stabilization of mRNA Display Shingo Ueno and Naoto Nemoto Part IV: Applications of Ribosome Display Methods Using Natural Amino Acids 9. Optimization of Antibody Affinity by Ribosome Display Using Error-Prone or Site-Directed Mutagenesis Leeanne Lewis and Chris Lloyd 10. Affinity Maturation of Phage Display Antibody Populations using Ribosome Display Maria A. Groves and Adrian A. Nickson 11. Evolution of Protein Stability Using Ribosome Display Andrew Buchanan 12. Selection of Lead Antibodies from Naive Ribosome Display Antibody Libraries Peter Ravn 13. Evolution of Disulfide-Rich Peptide Aptamers Using cDNA Display Yuki Mochizuki and Naoto Nemoto 14. Peptide Screening Using PURE Ribosome Display Hiroyuki Ohashi, Takashi Kanamori, Eriko Osada, Bintang K. Akbar, and Takuya Ueda 15. Rapid Selection of High Affinity Binders Using Ribosome Display Birgit Dreier and Andreas Pluckthun 16. mRNA Display-Based Selections Using Synthetic Peptide and Natural Protein Libraries Steve W. Cotten, Jianwei Zou, Rong Wang, Bao-cheng Huang, and Rihe Liu 17. Identification of Candidate Vaccine Genes Using Ribosome Display Liancheng Lei 18. Ribosome Display for the Selection of Sac7d Scaffolds Barbara Mouratou, Ghislaine Behar, Lauranne Paillard-Laurance, Stephane Colinet, and Frederic Pecorari Part V: Incorporation of Non-Natural Amino Acids for Selection by Ribosome Display and Related Methods 19. Charging of tRNAs Using Ribozymes and Selection of Cyclic Peptides Containing Thioethers Patrick C. Reid, Yuki Goto, Takayuki Katoh, and Hiroaki Suga 20. Update on Pure Translation Display with Unnatural Amino Acid Incorporation R. Edward Watts and Anthony C. Forster 21. In Vitro Selection of Unnatural Cyclic Peptide Libraries Via mRNA Display Zhong Ma and Matthew C.T. Hartman Part VI: Case Studies 22. Optimization of CAT-354, a Therapeutic Antibody Directed Against Interleukin-13, Using Ribosome Display George Thom and Ralph Minter 23. Affinity Maturation and Functional Dissection of a Humanized Anti-RAGE Monoclonal Antibody by Ribosome Display Simon E. Hufton