Septins

1. The Non-Opisthokont Septins: How Many There Are, How Little We Know About Them, and How We Might Learn More Masayuki Onishi and John R. Pringle 2. Preparing Recombinant Yeast Septins and Their Analysis by Electron Microscopy Aurelie Bertin and Eva Nogales 3. A FRET-Based Method for Monitoring Septin Polymerization and Binding of Septin-Associated Proteins Elizabeth A. Booth and Jeremy Thorner 4. In Vitro Reconstitution of Septin Assemblies on Supported Lipid Bilayers Andrew A. Bridges and Amy S. Gladfelter 5. Visualization of In Vivo Septin Ultrastructures by Platinum Replica Electron Microscopy Katy Ong, Tatyana Svitkina and Erfei Bi 6. Assays for Genetic Dissection of Septin Filament Assembly in Yeast, From De Novo Folding Through Polymerization Michael A. McMurray 7. Investigation of Septins Using Infection by Bacterial Pathogens Sina Krokowski and Serge Mostowy 8. In Vivo Analysis of Septin Heteropolymer Rods and Higher-Order Structures in Filamentous Fungi Amy Smith and Michelle Momany 9. Live Cell Imaging of Septin Dynamics in Ustilago maydis Sebastian Baumann, Sabrina Zander, Stefanie Weidtkamp-Peters and Michael Feldbrugge 10. Ashbya gossypii as a Model System to Study Septin Organization by Single-Molecule Localization Microscopy Charlotte Kaplan, Chenlu Yu and Helge Ewers 11. Visualizing Septins in Early Drosophila Embryos Manos Mavrakis 12. Purification of Recombinant Human and Drosophila Septin Hexamers for TIRF Assays of Actin-Septin Filament Assembly Manos Mavrakis, Feng-Ching Tsai and Gijsje H. Koenderink 13. Investigation of Septin Biology In Vivo Using Zebrafish Alexandra Willis, Maria Mazon Moya and Serge Mostowy 14. Fluorescence Microscopy of Actin- and Microtubule-Associated Septins in Mammalian Cells Elias T. Spiliotis, Eva P. Karasmanis and Lee Dolat 15. Immunofluorescent Staining of Septins in Primary Cilia Moshe S. Kim, Carol D. Froese, Hong Xie and William S. Trimble 16. Methods for Immunoblot Detection and High-Resolution Subcellular Mapping of Septin Subunits in Mammalian Nervous Systems Laxmi Kumar Parajuli, Natsumi Ageta-Ishihara, Hiroshi Ageta, Yugo Fukazawa and Makoto Kinoshita 17. Visualizing Septin and Cell Dynamics in Mammalian Brain Slices Hidenori Ito, Rika Morishita, Hidenori Tabata and Koh-ichi Nagata 18. Small Molecule Perturbations of Septins Lydia R. Heasley and Michael A. McMurray 19. Septin Crystallization for Structural Analysis Napoleao Fonseca Valadares and Richard Charles Garratt