Single Cell Diagnostics

Table of Contents 1. Non-invasive metabolic assessment of single cells David K. Gardner 2. Laser Capture Microdissection for Analysis of Single Cells Nobuki Nakamura, Katharina Ruebel, Long Jin, Xiang Qian, Heyu Zhang, and Ricardo V. Lloyd 3. Fluorescence in situ hybridization (FISH) on Single Cells (Sex Determination and Chromosome Rearrangements) Paul N. Scriven and Caroline Mackie Ogilvie 4. Single cell PCR for PGD: methods, strategies and limitations Karen Sermon and Martine De Rycke 5. Real-Time Quantitative PCR Measurement of Male Fetal DNA in maternal plasma Bernhard Zimmermann, Ahmad El-Sheikhah, Kypros Nicolaides, Wolfgang Holzgreve, Sinuhe Hahn 6. Cell-Free Fetal DNA Plasma Extraction and Real Time-PCR Quantification Jill L. Maron, Kirby L. Johnson, and Diana W. Bianchi 7. LATE-PCR and allied technologies: Real-time detection strategies for rapid, reliable diagnosis from single cells Kenneth E. Pierce and Lawrence J. Wangh 8. Efficient isothermal amplification of the entire genome from single cells Karen V. Schowalter, Jolene R. Fredrickson and Alan R Thornhill 9. Comparative Genomic Hybridization on single cells Lucille Voullaire and Leeanda Wilton 10. Generation of amplified RNAs and cDNA libraries from single mammalian cells James Adjaye 11. Real-time PCR to measure gene expression in single cells Dagan Wells 12. Gender Determination and Detection of Aneuploidy in Single Cells Using DNA Array-Based Comparative Genomic Hybridisation (Array CGH) Dong Gui Hui, Xin Yuan Guan, and Nicole Hussey 13. Microarray technology for mutation analysis of low template DNA samples Chelsea Salvado and David Cram