Sphingosine-1-Phosphate

1. Biochemical Methods for Quantifying Sphingolipids: Ceramide, Sphingosine, Sphingosine Kinase-1 Activity, and Sphingosine-1-Phosphate Brizuela Leyre and Cuvillier Olivier 2. Isoform-Selective Assays for Sphingosine Kinase Activity Melissa R. Pitman, Duyen H. Pham, and Stuart M. Pitson 3. Quantification of Sphingosine-1-Phosphate and Related Sphingolipids by Liquid Chromatography Coupled to Tandem Mass Spectrometry Constantin Bode and Markus H. Graler 4. A Clean-up Method for Mass Spectrometric Analysis of Sphingosine-1-Phosphate in Blood and Solid Tissues Using a Phosphate Capture Molecule Jun-ichi Morishige, Tamotsu Tanaka, and Kiyoshi Satouchi 5. Immunohistochemical Detection of Sphingosine-1-Phosphate and Sphingosine Kinase-1 in Human Tissue Samples Barbara Visentin, Gary Reynolds, and Roger Sabbadini 6. Assessment of Sphingosine-1-Phosphate Activity in Biological Samples by Receptor Internalization and Adherens Junction Formation Hideru Obinata and Timothy Hla 7. High Throughput Assays to Measure Intracellular Ca2+ Mobilization in Cells that Express Recombinant S1P Receptor Subtypes William J. Valentine and Gabor Tigyi 8. Quantifying Sphingosine-1-Phosphate-Dependent Activation of the RhoGTPases Matt D. Medlin, Joan M. Taylor, and Christopher P. Mack 9. Optimized Protocols to Analyze Sphingosine-1-Phosphate Signal Transduction Pathways During Acrosomal Exocytosis in Human Sperm Silvia A. Belmonte and Laila Suhaiman 10. Use of Intravital Microscopy and In Vitro Chemotaxis Assays to Study the Roles of Sphingosine-1-Phosphate in Bone Homeostasis Taeko Ishii, Shunsuke Kawamura, Issei Nishiyama, Junichi Kikuta, and Masaru Ishii 11. Assessment of Sphingosine-1-Phosphate Receptor Expression and Associated Intracellular Signaling Cascades in Primary Cells of the Human Central Nervous System Veronique E. Miron, Bryce A. Durafourt, Jack P. Antel, and Timothy E. Kennedy 12. Sphingosine-1-Phosphate Signaling in Skeletal Muscle Cells Elisabetta Meacci, Francesca Bini, and Chiara Battistini 13. Maintenance of Human Embryonic Stem Cells by Sphingosine-1-Phosphate and Platelet-Derived Growth Factor Raymond C.B. Wong, Martin F. Pera, and Alice Pebay 14. Ceramide and Sphingosine-1-Phosphate Signaling in Embryonic Stem Cell Differentiation Erhard Bieberich 15. Sphingosine-1-Phosphate: An Alternative to Serum for Culture and Multipotency Maintenance of Adult Human Mesenchymal Stem Cells He Xiaoli, Sathish Jean, and Alirio J. Melendez 16. Sphingosine-1-Phosphate Signaling in Neural Progenitors Phillip Callihan and Shelley B. Hooks 17. Utilizing Sphingosine-1-Phosphate to Stimulate Sprouting Angiogenesis Shih-Chi Su and Kayla J. Bayless