LABORATORY PROCEDURES 1 The documentation system Markus Montag and Lars Johansson 2 Culture Media Techniques Lars Johansson 3 IVF incubator handling Jason E Swain and Alex Lagunov 4 Preparation for follicle aspiration and isolation of cumulus-oocyte-complexes (COC) Markus Montag 5 Semen collection and sample reception Carin Huyser 6 Spermiogramm, Makler chamber, Neubauer chamber, CASA Verena Nordhoff 7 Sperm viability testing Markus Montag 8 Sperm preparation - Swim up, density gradient, migration chamber, zeta method Thomas Ebner 9 Sperm selection using hyaluronan binding Denny Sakkas 10 IVF - Insemination Markus Montag 11 ICSI Thomas Freour 12 Oocyte in vitro maturation - Isolation of COCs and culture for maturation Mariabeatrice Dal Canto and Giovanni Coticchio 13 Artificial oocyte activation Markus Montag 14 Cryopreservation - Sperm TESE and MESA Borut Kovacic 15 Vitrification - Oocytes, 2PN, Embryos, Blastocysts Ana Cobo and Aila Coello 16 Slow freezing: principles, oocytes, 2PN, embryos, blastocysts Etienne Van den Abbeel 17 Oocyte selection Basak Balaban 18 Zygote selection Martin Greuner 19 Selection and grading of embryos on day 2/3 and blastocysts on day 5/6 Thorir Hardarson and Julius Hreinsson 20 Time-lapse imaging Cristina Hickman 21 Assisted hatching Maria De Geyter and Christian De Geyter 22 Zona manipulation at various embryo stages: Mechanical / acidic medium Marius Meintjes 23 Biopsy: polar body, blastomere, trophectoderm Hakan Kadir Yelke and Semra Kahraman 24 Sampling of cells after biopsy for aCGH Barry Behr 25 Embryo transfer - standard and ultrasound guided Bettina Toth 26 Hygenic standards in the IVF laboratory Vera Baukloh 27 Quality control of IVF laboratory supplies - Mouse Embryo Assay (MEA) Dean E Morbeck 28 Troubleshooting in the IVF laboratory David Mortimer and Sharon T Mortimer 29 Microscopy Markus Montag 30 Fertility preservation Markus Montag and Jana Liebenthron CLINICAL PROCEDURES 31 Ultrasound evaluation in treatment management Ippokratis Sarris and Geeta Nargund 32 Three-Dimensional Ultrasound and the Diagnosis of Uterine Anomalies Magued Adel Aziz Mikhail, Megan Lively, Candice P. Holliday, and Botros Rizk 33 Infertility ultrasound evaluation: One Stop Procedure Spiros A. Liatsikos and Geeta Nargund 34 Office Hysteroscopy Linda D. Bradley 35 Hysteroscopic management of uterine septum Omar Abuzeid and Mostafa Abuzeid 36 Hysteroscopic Lysis of Intrauterine Adhesions Bolonduro Oluwamuyiwa and Mostafa Abuzeid 37 Hysteroscopic Management of Submucosal Fibroids Salem Joseph and Mostafa Abuzeid 38 Saline infusion sonohysterography Alan Bolnick and Mostafa Abuzeid 39 General Principles of Laparoscopy Rubin Raju and Mostafa Abuzeid 40 Microlaparoscopy Oscar D. Almeida Jr 41 Laparoscopic myomectomy and minilaparotomy: an alternative approach for myomectomy Mostafa Abuzeid and Ahmed Abdel Aziz 42 Laparoscopic Excision of a Large Endometrioma Rubin Raju and Mostafa Abuzeid 43 Laparoscopic management of subtle fimbrial pathology secondary to endometriosis Omar Abuzeid and Mostafa Abuzeid 44 Laparoscopic management of mature cystic teratoma Rahima Sanya and Mostafa Abuzeid 45 Tubal Cannulation for Proximal Tubal Disease Botros Rizk and Josh Ekladios 46 Robotic assisted laparoscopic myomectomy Hashem Lofty, Arsany Bassily, Lauren Mann, and Botros Rizk 47 Robotic Assisted Tubal Anastomosis After Tubal Sterilization Hashem Lofty, Rola Turki, and Botros Rizk 48 Unexplained infertility Hassan N. Sallam and Nooman H. Sallam 49 Male Evaluation for Infertility Kiranpreet Khurana, Edmund Sabanegh, Jr., and Ashok Agarwal 50 Hysterosalpingography Islam Fahmi, Mostafa Abuzeid, and Shawky ZA Badawy 51 Intrauterine insemination: clinical perspectives Willem Ombelet 52 GnRH agonist to trigger final follicular maturation in IVF Nayana Talukdar and Robert F. Casper 53 GnRH agonist Protocols Justin Tan, Rabea Youcef Khoudja, Michael Doley, Michael Dahan and Seang Lin Tan 54 GnRH agonists before IVF for advanced endometriosis Hassan N. Sallam and Nooman H. Sallam 55 Microdose GnRH Agonist Flare Protocols Magued Adel Mikhail, Wael Salem, and Botros Rizk 56 Gonadotropin Releasing Hormone Antagonist Cycle for In Vitro Fertilization Michael Dahan, Justin Tan, Baris Ata, Rabea Khoudja, Seang Lin Tan, and Botros Rizk 57 Minimal stimulation for IVF Ippokratis Sarris and Geeta Nargund 58 Ovarian Hyperstimulation Syndrome Magued Adel Mikhail, Daniel Antonious, Candice P. Holliday, and Botros Rizk 59 The Prediction of OHSS Jan Gerris and Jana Claeys 60 Tips on technique of embryo transfer Omar Abuzeid and Mostafa Abuzeid 61 Difficult Embryo Transfers Gautam N. Allahbadia and Goral N. Gandhi 62 In vitro maturation of oocytes for fertility treatment and fertility preservation Michael H. Dahan, Justin Tan, Rabea Youcef Khoudja, Andrew Mok, and Seang Lin Tan 63 Complications and outcomes of IVF Jan Gerris 64 Obstetric complications after in-vitro fertilization Brian Brocato and David Lewis 65 Twins pregnancy after ART David F. Lewis and Brian Brocato 66 IVF and endometriosis Graciela Kohls Ilgner and Juan Antonio Garcia Velasco 67 Leiomyoma of the Uterus Shawky ZA Badawy 68 Office Management of Polycystic Ovarian Syndrome Shawky ZA Badawy 69 Adenomyosis: Imaging and Treatment Magued Adel Mikhail, Candice P. Holliday, and Botros Rizk 70 Ectopic Pregnancy: Evaluation and Management James P. Schwayder