Testing Methods for Seed-Transmitted Vi

Principles and Protocols

By: Sven Albrechtsen

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Spiral Ringed Book | 5 January 2006

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Spiral Ringed Book
268 Pages

Dimensions(cm)
24.13 x 18.42 x 1.91

Spiral Ringed Book

$227.25

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This practical guide covers the commonly used detection methods for seed-transmitted viruses and viroids that affect both tropical and temperate crops. It contains 25 complete step-by-step procedures for biological, serological and molecular techniques to detect and identify such viruses. Combining helpful practical notes with more detailed explanations of the principles behind the techniques, the book describes the general characteristics of seed-transmitted viral diseases and discusses outlines for the organization and interpretation of seed health assays. The techniques reviewed are also applicable to non-seed-transmitted viral agents.

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Preface
Acknowledgements

Introduction
Seed-transmitted Viruses and Viroids
The Development of the Science
Seed-health testing for viruses
Economic Importance of Seed-transmitted Viruses
Test of Seeds for Viruses: Why?
Seed Certification
Quarantine
References
Seed Transmission of Viruses
Location of Inoculum in the Seed
Plant-to-seed and Seed-to- plant Transmission
Plant-to-seed transmission
Seed-to-plant transmission
Factors affecting seed transmission
References
Ecology, Epidemiology and Control
Ecology and Epidemiology
Viruses and virus-host interaction
Vector Transmission
Environment and cultural practices
Control Strategies
Control measures at farm level
Control measures at research and technology level
References

Biological Assays
Symptomatology
Indicator plant symptoms
Facilities and Equipment for Biological Assays
Growth Facilities
Plant Culture materials and equipment
Growing-on Tests
Growing-on test, standard protocol
Infectivity
Production of indicator plants
Inoculation techniques and standard protocol
Seed as inoculum, protocols
Plants and seedlings as inoculum, protocol
Differential hosts
Maintaining of Isolates
Long-term storage of dehydrated samples, protocol
References
Serological Testing Methods
Antigens and Anitbodies
Polyclonal antibodies
Monoclonal antibodies
Enzyme-linked Immunosorbent Assay (ELISA)
General principles of ELISA
General components and steps of ELISA
Double-antibody sandwich ELISA (DAS-ELISA), protocols
Indirect antigen-first (AgF) ELISA, protocol
Triple-antibody sandwich ELISA (TAS-ELISA)
Penicillinase-ELISA, protocol
Other variants of ELISA
Remarks on ELISA systems, including protocol for plate reuse
Recording and interpretation
Dot Immunobinding Assay (DIBA)
Principles of DIBA
General components and steps of DIBA
Indirect AgF-DIBA, protocol
Tissue Blotting Immunoassay (TBIA)
Tissue blotting immunoassay (TBIA), protocol
Other Serological Test Methods
References
Nucleic Acid-Based Testing Methods
Nucleic Acid Hybridization
General principles and components of nucleic
Non-isotopic nucleic acid spot hybridization, protocol
Enzymatic Nucleic Acid Amplification
Principle of the polymerase chain reaction (PCR)
General components of the reverse-transcription polymerase chain reaction (RT-PCR) assay
Protocols for RT-PCR assays
Analysis of PCR products and protocol
Detection of virus species and strains
Variants of molecular amplification assays
Remarks on molecular amplification
References
Epilogue
Other Detection Techniques
Electron microscopy
Table of Contents provided by Publisher. All Rights Reserved.

Testing Methods for Seed-Transmitted Vi

Principles and Protocols

At a Glance

Spiral Ringed Book

Shipping

How to return your order

Defective items

You Can Find This Book In

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