The Mitotic Exit Network

Part I: Analysis of Cell Cycle Progression and Mitotic Exit 1. Regulation of Mitotic Exit in Saccharomyces cerevisiae Barbara Baro, Ethel Queralt, and Fernando Monje-Casas 2. Methods of Synchronization of Yeast Cells for the Analysis of Cell Cycle Progression M. Angeles Juanes 3. Analysis of Cell Cycle Progression in Saccharomyces cerevisiae Using the Budding Index and Tubulin Staining Marta Munoz-Barrera and Fernando Monje-Casas 4. Determination of Cell Cycle Stage and Mitotic Exit Through the Quantification of the Protein Levels of Known Mitotic Regulators Cristina Cepeda-Garcia 5. Cdc14 Localization as a Marker for Mitotic Exit: In Vivo Quantitative Analysis of Cdc14 Release Gabriel Neurohr and Manuel Mendoza Part II: Biochemistry of the MEN 6. In Vitro Analysis of Tem1 GTPase Activity and Regulation by the Bfa1/Bub2 GAP Marco Geymonat, Adonis Spanos, and Katrin Rittinger 7. Analysis of Protein-Protein Interactions Between MEN Components Rami Rahal 8. A New Methodology for the Quantification of In Vivo Cdc14 Phosphatase Activity Ethel Queralt and Jose-Antonio Rodriguez-Rodriguez 9. Analysis of SUMOylation in the RENT Complex by Fusion to a SUMO-Specific Protease Domain Neus Colomina, Claudia Guasch, and Jordi Torres-Rosell 10. A Substrate Trapping Method for Identification of Direct Cdc14 Phosphatase Targets Brendan L. Powers, Hana Hall, Harry Charbonneau, and Mark C. Hall Part III: Analysis of the Localization of MEN Components 11. Localizing MEN Components by Indirect Immunofluorescence Analysis of Budding Yeast Francesca Senic-Matuglia and Rosella Visintin 12. Analysis of the Localization of MEN Components by Live Cell Imaging Microscopy Zhiang Guo and Marisa Segal 13. Evaluation of the Dynamicity of Mitotic Exit Network and Spindle Position Checkpoint Components on Spindle Pole Bodies by Fluorescence Recovery After Photobleaching (FRAP) Ayse Koca Caydasi and Gislene Pereira 14. Asymmetric Localization of Components and Regulators of the Mitotic Exit Network at Spindle Pole Bodies Ilaria Scarfone and Simonetta Piatti 15. Evaluation of the Nucleolar Localization of the RENT Complex to Ribosomal DNA by Chromatin Immunoprecipitation Assays Julie Huang, Nahid Iglesias, and Danesh Moazed Part IV: Control of Cellular Processes by the MEN 16. Analysis of the Functionality of the Mitotic Checkpoints Roberta Fraschini 17. Cdc14 and Chromosome Condensation: Evaluation of the Recruitment of Condensin to Genomic Regions Facundo Ramos, Joanne Leonard, Andres Clemente-Blanco, and Luis Aragon 18. Studying the Role of the Mitotic Exit Network in Cytokinesis Magdalena Foltman and Alberto Sanchez-Diaz Part V: Final Remarks: The MEN as a Model for Higher Eukaryotes 19. Hippo Signaling in Mitosis: An Updated View in Light of the MEN Pathway Alexander Hergovich