PART I: Molecular Characterization 1. Analysis of Chromosomal Alterations in Urothelial Carcinoma Donatella Conconi and Angela Bentivegna 2. Analysis of Point Mutations in Clinical Samples of Urothelial Carcinoma Mustafa Alamyar and Ellen C. Zwarthoff 3. A Versatile Assay for Detection of Aberrant DNA Methylation in Bladder Cancer Stella Tommasi and Ahmad Besaratinia 4. Immunohistochemical Analysis of UC Tissues for Proliferation and Differentiation Markers Michael Rose and Nadine Therese Gaisa 5. Molecular Subtype Profiling of Urothelial Carcinoma Using a Subtype-Specific Immunohistochemistry Panel Gottfrid Sjodahl PART II: Urothelial Carcinogenesis 6. Defining the Pathways of Urogenital Schistosomiasis-associated Urothelial Carcinogenesis through Transgenic and Bladder Wall Egg Injection Models Evaristus C. Mbanefo and Michael H. Hsieh < 7. Algorithm for the Automated Evaluation of NAT2 Genotypes Georg Michael, Ricarda Thier, Meinolf Blaszkewicz, Silvia Selinski, and Klaus Golka 8. Detection of APOBEC3 Proteins and Catalytic Activity in Urothelial Carcinoma"br> Ananda Ayyappan Jaguva Vasudevan, Wolfgang Goring, Dieter Haussinger, and Carsten Munk 9. Oxidative Stress in Urothelial Carcinogenesis: Measurements of Protein Carbonylation and Intracellular Production of Reactive Oxygen Species Patcharawalai Whongsiri, Suchittra Phoyen, and Chanchai Boonla PART III: Cellular and ANIMAL MODELS 10. Urothelial Carcinoma Stem Cells - current Concepts, Controversies, and Methods Jiri Hatina, Hamendra Singh Parmar, Michaela Kripnerova, Anastasia Hepburn, and Rakesh Heer 11. <In Vitro Differentiation and Propagation of Urothelium from Pluripotent Stem Cell Lines Stephanie L. Osborn and Eric A. Kurzrock 12. Spheroid Cultures of Primary Urothelial Cancer Cells: Cancer Tissue-originated Spheroid (CTOS) Method Takahiro Yoshida, Hiroaki Okuyama, Hiroko Endo, and Masahiro lnoue 13. The N-butyl-N-4-hydroxybutyl Nitrosamine Mouse Urinary Bladder Cancer Model Paula Alexandra Oliveira, Carmen Vasconcelos-Nobrega, Rui Manuel Gil da Costa, and Regina Arantes-Rodrigues 14. Patient-derived Bladder Cancer Xenografts Carina Bernardo and Lucio Lara Santos 15.< Orthotopic Mouse Models of Urothelial Cancer Wolfgang Jager, Igor Moskalev, Peter Raven, Akihiro Goriki, Samir Bidnur, and Peter C. Black PART IV: Biomarkers 16. Quantification of microRNAs in Urine-derived Specimens Susanne Fuessel, Andrea Lohse-Fischer, Dana Vu Van, Karsten Salomo, Kati Erdmann, and Manfred P. Wirth 17. Quantitative RNA Analysis from Urine using Real Time PCR Lourdes Mengual and Mireia Olivan 18. DNA Methylation Analysis from Body Fluids Dimo Dietrich 19. Urinary Protein Markers for the Detection and Prognostication of Urothelial Carcinoma Tibor Szarvas, Peter Nyirady, Takashi Kobayashi, Osamu Ogawa, Hideki Furuya, and Charles J. Rosser 20. Isolation and Characterization of CTCs from Patients with Cancer of a Urothelial Origin Vladimir Bobek and Katarina Kolostova PART V: Therapy Development 21. Epigenetic Treatment Options in Urothelial Carcinoma Guenter Niegisch, Maria Pinkerneil, and Michele Hoffmann 22. Evaluation of Protein Levels of the Receptor Tyrosine Kinase ErbB3 in Serum Leandro S. D'Abronzo, Chong-Xian Pan, and Paramita M. Ghosh 23. Targeting the PI3K/AKT/mTOR Pathway in Bladder Cancer Anuja Sathe and Roman Nawroth 24. Visualization and Quantitative Measurement of Frug-induced Platinum Sdducts in the Nuclear DNA of Individual Cells by an Immuno-cytological Sssay Margarita Melnikova and Jurgen Thomale
