Xenopus Protocols

Part I: Xenopus Tropicalis 1. Xenopus tropicalis as a Model Organism for Genetics and Genomics: Past, Present and FutureRobert M. Grainger2. Husbandry of Xenopus tropicalis Alan Jafkins, Anita Abu-Daya, Anna Noble, Lyle Zimmerman, and Matthew Guille3. Generating Diploid Embryos from Xenopus tropicalisFlorencia del Viso and Mustafa Khokha4. Navigating the Xenopus tropicalis GenomeIra L. BlitzPart II: Genetics in Xenopus5. Genetic Analysis of Xenopus tropicalisTimothy J. Geach, Derek L. Stemple, and Lyle B. Zimmerman6. Forward Genetic Screens in Xenopus using Transposon-mediated Insertional Mutagenesis Donald A. Yergeau, Clair M. Kelley, Haiqing Zhu, Emin Kuliyev and Paul E. Mead7. Targeted Gene Disruption with Engineered Zinc-finger Nucleases (ZFNs)John J. Young and Richard M. Harland 8. Reverse Genetic Studies using Antisense Morpholino Oligonucleotides Yanan Zhao, Shoko Ishibashi and Enrique Amaya9. Chemical Genetics and Drug Discovery in XenopusMatthew L. Tomlinson, Adam Hendry, and Grant N. Wheeler10. Maternal mRNA Knock-down Studies: Antisense Experiments using the Host-transfer Technique in Xenopus laevis and Xenopus tropicalis David J. Olson, Alissa M. Hulstrand and Douglas W. HoustonPart II: Xenopus Trangenesis11. Generating Transgenic Frog Embryos by Restriction Enzyme Mediated Integration (REMI)Shoko Ishibashi, Kristen L. Kroll and Enrique Amaya 12. A Simple Method of Transgenesis using I-SceI meganuclease in XenopusShoko Ishibashi, Nick R. Love and Enrique Amaya13. Using FC31 Integrase to Mediate Insertion of DNA in Xenopus EmbryosYou E. Li, Bryan G. Allen and Daniel L. Weeks14. Xenopus Transgenics: Methods using Transposons Clair M. Kelley, Donald A. Yergeau, Haiqing Zhu, Emin Kuliyev and Paul E. Mead15. Comparative Genomics-based Identification and Analysis of Cis-regulatory ElementsHajime Ogino, Haruki Ochi, Chihiro Uchiyama, Sarah Louie, and Robert M. Grainger16. Tet-On Binary Systems for Tissue-specific andInducible Transgene ExpressionDaniel R. BuchholzPart III: Gene Expression: From Gene to Transcript to Protein17. Chromatin Immunoprecipitation Analysis of Xenopus Embryos Robert C. Akkers, Ulrike G. Jacobi and Gert Jan C. Veenstra18. Transcriptomics Using Next Generation Sequencing TechnologiesDasfne Lee-Liu, Leonardo I. Almonacid, Fernando Faune, Francisco Melo, Juan Larrain 19. Databases of Gene Expression in Xenopus DevelopmentMichael J. Gilchrist, Nicolas Pollet20. Investigating Alternative RNA Splicing in Xenopus Agnes Mereau and Serge Hardy21. Immunoisolation of Protein Complexes from XenopusFrank L. Conlon, Yana Miteva, Erin Kaltenbrun, Lauren Waldron, Todd M. Greco, Ileana M. Cristea 22. Complementary Proteomic Analysis of Protein Complexes Todd M. Greco, Yana Miteva, Frank L. Conlon, Ileana M. Cristea Part IV: Imaging Xenopus Development 23. Antibody Development and use in Chromogenic and Fluorescent ImmunostainingEamon Dubaissi, Niki Panagiotaki, Nancy Papalopulu, and Peter D. Vize24. Multiple fluorescent in situ mRNA Hybridization (FISH) on Whole Mounts and SectionsRobert Lea, Boyan Bonev, Eamon Dubaissi, Peter D. Vize and Nancy Papalopulu25. Methods to Analyze microRNA Expression and Function During Xenopus Development Boyan Bonev and Nancy Papalopulu26. A Bromodeoxyuridine (BrdU) Based Protocol for Characterizing Proliferating Progenitors in Xenopus Embryos Helene Auger, Raphael Thuret, Warif El Yakoubi, and Nancy Papalopulu27. Microscopy Tools for Quantifying Developmental Dynamics in Xenopus EmbryosSagar D. Joshi, Hye Young Kim, Lance A. DavidsonPart V: Novel Approaches in Xenopus28. Mathematical Modelling of Gene RegulatoryNetworks in Xenopus DevelopmentYasushi Saka29. Stem-cell-like Embryonic Explants to Study Cardiac DevelopmentBoni A. Afouda30. Studying Regeneration in XenopusCaroline W. Beck31. On-line Resources for Xenopus Jeff BowesBoyan Bonev and Nancy Papalopulu