Part I: Larval Zebrafish Models 1. Measuring Larval Zebrafish Behavior: Locomotion, Thigmotaxis, and Startle William H.J. Norton 2. The Light-Dark Preference Test for Larval Zebrafish Peter J. Steenbergen, Michael K. Richardson, and Danielle L. Champagne 3. Assessment of Thigmotaxis in Larval Zebrafish Stephanie J. Schnorr, Peter J. Steenbergen, Michael K. Richardson, and Danielle L. Champagne 4. Zebrafish and Drug Development: A Behavioral Assay System for Probing Nicotine Function in Larval Zebrafish Henning Schneider, Eric W. Klee, Karl J. Clark, Andrew M. Petzold, Vanessa L. Mock, Julia M. Abarr, Jennifer L. Behrens, Ryan E. Edelen, Bryan A. Edwards, Joshua S. Hobgood, Meghann E. Pogue, Nishant K. Singh, and Stephen C. Ekker 5. Olfactory Evoked Activity Assay for Larval Zebrafish Ganive Bhinder and Keith B. Tierney 6. Qualitative and Quantitative Measurements of the Optokinetic Response in Larval Zebrafish Kaspar P. Mueller and Stephan C.F. Neuhauss 7. Automated Imaging of Visual Recognition Memory in Larval Zebrafish Ruth M. Colwill and Robbert Creton 8. Automated Conditioning in Larval Zebrafish Ruey-Kuang Cheng and Suresh Jesuthasan 9. A Method to Quantify Basal and Stress-Induced Cortisol Response in Larval Zebrafish Peter J. Steenbergen, Juriaan R. Metz, Gert Flik, Michael K. Richardson, and Danielle L. Champagne Part II: Adult Zebrafish Models 10. A Method for Sorting Zebrafish on the Exploratory-Bold Behavioral Axis Cory D. Sailer, Sonny J. Radenic, and Brian D. Wisenden 11. Nocifensive Behavior in Adult and Larval Zebrafish Monica Gomes Lima, Caio Maximino, and Anderson Manoel Herculano 12. Use of a Split Depth and Visual Cliff Apparatus to Measure the Diving Response in Zebrafish Rachel Blaser and Kelly Goldsteinholm 13. Adapting the Open Field Test to Assess Anxiety-Related Behavior in Zebrafish John Godwin, Susanna Sawyer, Florence Perrin, Sarah E. Oxendine, and Zachary D. Kezios 14. Behavioral and Pharmacological Aspects of Anxiety in the Light/Dark Preference Test Juliana Araujo, Caio Maximino, Thiago Marques de Brito, Annanda Waneza Batista da Silva, Silvio Morato, Anderson Manoel Herculano, and Amauri Gouveia, Jr. 15. A High Throughput and Inexpensive Assay for Anxiety-Related Behaviors in the Zebrafish, Based on Place Preference and Latency to Feed Barrie D. Robison, Maia J. Benner, Matthew L. Singer, and Mary E. Oswald 16. Automated Tracking of Zebrafish Shoals and the Analysis of Shoaling Behavior Noam Miller and Robert Gerlai 17. Assessing Social Behavior Phenotypes in Adult Zebrafish: Shoaling, Social Preference, and Mirror Biting Tests Mimi Pham, Jolia Raymond, Jonathan Hester, Evan Kyzar, Siddharth Gaikwad, Indya Bruce, Caroline Fryar, Simon Chanin, Joseph Enriquez, Sidarth Bagawandoss, Ivan Zapolsky, Jeremy Green, Adam Michael Stewart, Barrie D. Robison, and Allan V. Kalueff 18. Measuring Effects of Psychostimulants on Egocentric Spatial Learning and Memory in Adult Zebrafish Rabia Nasir, Tenzin Choezom, Jennifer Cunningham, Benjamin Bajaj, Cristina Rubi, Jonathan Butler, Kathryn Arroyo, and Joseph A. Schroeder 19. Cued Fear Conditioning in Zebrafish (Danio rerio) Masakazu Agetsuma, Tazu Aoki, Ryo Aoki, and Hitoshi Okamoto 20. Assessing Learning and Memory Through the Active Avoidance Paradigm Xiaojuan Xu and Stefan Goetz 21. Assessing Habituation Phenotypes in Adult Zebrafish: Intra- and Inter-Trial Habituation in the Novel Tank Test Jolia Raymond, Simon Chanin, Adam Michael Stewart, Evan Kyzar, Siddharth Gaikwad, Andrew Roth, Indya Bruce, Caroline Fryar, Danielle Varga, Joseph Enriquez, Sidarth Bagawandoss, Mimi Pham, Ivan Zapolsky, Jeremy Green, Daniel Desmond, Jonathan Hester, and Allan V. Kalueff 22. Assessing Startle Responses and Their Habituation in Adult Zebrafish Simon Chanin, Caroline Fryar, Danielle Varga, Jolia Raymond, Evan Kyzar, Joseph Enriquez, Sidarth Bagawandoss, Siddharth Gaikwad, Andrew Roth, Mimi Pham, Ivan Zapolsky, Indya Bruce, Jonathan Hester, Jeremy Green, Daniel Desmond, Adam Michael Stewart, and Allan V. Kalueff 23. QTL Mapping Using Behavioral Traits in the Adult Zebrafish Dominic Wright 24. Assessing Epilepsy-Related Behavioral Phenotypes in Adult Zebrafish Daniel Desmond, Evan Kyzar, Siddharth Gaikwad, Jeremy Green, Russell Riehl, Andrew Roth, Adam Michael Stewart, and Allan V. Kalueff 25. Rapid Method for Acute Intracerebroventricular Injection in Adult Zebrafish Augusto Barbosa, Jr., Caio Maximino, Aparecida de Souza Fim Pereira, Carla Cristina Bejo Wolkers, Fabiana Luca Alves, Liliam Midori Ide, Anderson Manoel Herculano, and Anette Hoffmann 26. Western Blotting Assay for Egr-1 Immediate Early Gene in Brain Tissue of Zebrafish Applied to Neuroethological Study Augusto Barbosa, Jr., Anette Hoffmann, Fabiana Luca Alves, Carla Cristina Bejo Wolkers, Fernando Massaru Hoshiko, Liliam Midori Ide, Lilian Eslaine Costa Mendes Silva, and Paula Santos 27. Utilizing the Zebrafish Neurophenome Project (ZNP) Database for Analyses of Complex Neurophenotypes in Zebrafish Models Ivan Zapolsky, Evan Kyzar, Jeremy Green, Siddharth Gaikwad, Mimi Pham, Simon Chanin, Caroline Fryar, Jonathan Hester, Sidarth Bagawandoss, Jolia Raymond, Joseph Enriquez, Adam Michael Stewart, and Allan V. Kalueff Ganive Bhinder and Keith B. Tierney 6. Qualitative and Quantitative Measurements of the Optokinetic Response in Larval Zebrafish Kaspar P. Mueller and Stephan C.F. Neuhauss 7. Automated Imaging of Visual Recognition Memory in Larval Zebrafish Ruth M. Colwill and Robbert Creton 8. Automated Conditioning in Larval Zebrafish Ruey-Kuang Cheng and Suresh Jesuthasan 9. A Method to Quantify Basal and Stress-Induced Cortisol Response in Larval Zebrafish Peter J. Steenbergen, Juriaan R. Metz, Gert Flik, Michael K. Richardson, and Danielle L. Champagne Part II: Adult Zebrafish Models 10. A Method for Sorting Zebrafish on the Exploratory-Bold Behavioral Axis Cory D. Sailer, Sonny J. Radenic, and Brian D. Wisenden 11. Nocifensive Behavior in Adult and Larval Zebrafish Monica Gomes Lima, Caio Maximino, and Anderson Manoel Herculano 12. Use of a Split Depth and Visual Cliff Apparatus to Measure the Diving Response in Zebrafish Rachel Blaser and Kelly Goldsteinholm 13. Adapting the Open Field Test to Assess Anxiety-Related Behavior in Zebrafish John Godwin, Susanna Sawyer, Florence Perrin, Sarah E. Oxendine, and Zachary D. Kezios 14. Behavioral and Pharmacological Aspects of Anxiety in the Light/Dark Preference Test Juliana Araujo, Caio Maximino, Thiago Marques de Brito, Annanda Waneza Batista da Silva, Silvio Morato, Anderson Manoel Herculano, and Amauri Gouveia, Jr. 15. A High Throughput and Inexpensive Assay for Anxiety-Related Behaviors in the Zebrafish, Based on Place Preference and Latency to Feed Barrie D. Robison, Maia J. Benner, Matthew L. Singer, and Mary E. Oswald 16. Automated Tracking of Zebrafish Shoals and the Analysis of Shoaling Behavior Noam Miller and Robert Gerlai 17. Assessing Social Behavior Phenotypes in Adult Zebrafish: Shoaling, Social Preference, and Mirror Biting Tests Mimi Pham, Jolia Raymond, Jonathan Hester, Evan Kyzar, Siddharth Gaikwad, Indya Bruce, Caroline Fryar, Simon Chanin, Joseph Enriquez, Sidarth Bagawandoss, Ivan Zapolsky, Jeremy Green, Adam Michael Stewart, Barrie D. Robison, and Allan V. Kalueff 18. Measuring Effects of Psychostimulants on Egocentric Spatial Learning and Memory in Adult Zebrafish Rabia Nasir, Tenzin Choezom, Jennifer Cunningham, Benjamin Bajaj, Cristina Rubi, Jonathan Butler, Kathryn Arroyo, and Joseph A. Schroeder 19. Cued Fear Conditioning in Zebrafish (Danio rerio) Masakazu Agetsuma, Tazu Aoki, Ryo Aoki, and Hitoshi Okamoto 20. Assessing Learning and Memory Through the Active Avoidance Paradigm Xiaojuan Xu and Stefan Goetz 21. Assessing Habituation Phenotypes in Adult Zebrafish: Intra- and Inter-Trial Habituation in the Novel Tank Test Jolia Raymond, Simon Chanin, Adam Michael Stewart, Evan Kyzar, Siddharth Gaikwad, Andrew Roth, Indya Bruce, Caroline Fryar, Danielle Varga, Joseph Enriquez, Sidarth Bagawandoss, Mimi Pham, Ivan Zapolsky, Jeremy Green, Daniel Desmond, Jonathan Hester, and Allan V. Kalueff 22. Assessing Startle Responses and Their Habituation in Adult Zebrafish Simon Chanin, Caroline Fryar, Danielle Varga, Jolia Raymond, Evan Kyzar, Joseph Enriquez, Sidarth Bagawandoss, Siddharth Gaikwad, Andrew Roth, Mimi Pham, Ivan Zapolsky, Indya Bruce, Jonathan Hester, Jeremy Green, Daniel Desmond, Adam Michael Stewart, and Allan V. Kalueff 23. QTL Mapping Using Behavioral Traits in the Adult Zebrafish Dominic Wright 24. Assessing Epilepsy-Related Behavioral Phenotypes in Adult Zebrafish Daniel Desmond, Evan Kyzar, Siddharth Gaikwad, Jeremy Green, Russell Riehl, Andrew Roth, Adam Michael Stewart, and Allan V. Kalueff 25. Rapid Method for Acute Intracerebroventricular Injection in Adult Zebrafish Augusto Barbosa, Jr., Caio Maximino, Aparecida de Souza Fim Pereira, Carla Cristina Bejo Wolkers, Fabiana Luca Alves, Liliam Midori Ide, Anderson Manoel Herculano, and Anette Hoffmann 26. Western Blotting Assay for Egr-1 Immediate Early Gene in Brain Tissue of Zebrafish Applied to Neuroethological Study Augusto Barbosa, Jr., Anette Hoffmann, Fabiana Luca Alves, Carla Cristina Bejo Wolkers, Fernando Massaru Hoshiko, Liliam Midori Ide, Lilian Eslaine Costa Mendes Silva, and Paula Santos 27. Utilizing the Zebrafish Neurophenome Project (ZNP) Database for Analyses of Complex Neurophenotypes in Zebrafish Models Ivan Zapolsky, Evan Kyzar, Jeremy Green, Siddharth Gaikwad, Mimi Pham, Simon Chanin, Caroline Fryar, Jonathan Hester, Sidarth Bagawandoss, Jolia Raymond, Joseph Enriquez, Adam Michael Stewart, and Allan V. Kalueff 10. A Method for Sorting Zebrafish on the Exploratory-Bold Behavioral Axis Cory D. Sailer, Sonny J. Radenic, and Brian D. Wisenden 11. Nocifensive Behavior in Adult and Larval Zebrafish Monica Gomes Lima, Caio Maximino, and Anderson Manoel Herculano 12. Use of a Split Depth and Visual Cliff Apparatus to Measure the Diving Response in Zebrafish Rachel Blaser and Kelly Goldsteinholm 13. Adapting the Open Field Test to Assess Anxiety-Related Behavior in Zebrafish John Godwin, Susanna Sawyer, Florence Perrin, Sarah E. Oxendine, and Zachary D. Kezios 14. Behavioral and Pharmacological Aspects of Anxiety in the Light/Dark Preference Test Juliana Araujo, Caio Maximino, Thiago Marques de Brito, Annanda Waneza Batista da Silva, Silvio Morato, Anderson Manoel Herculano, and Amauri Gouveia, Jr. 15. A High Throughput and Inexpensive Assay for Anxiety-Related Behaviors in the Zebrafish, Based on Place Preference and Latency to Feed Barrie D. Robison, Maia J. Benner, Matthew L. Singer, and Mary E. Oswald 16. Automated Tracking of Zebrafish Shoals and the Analysis of Shoaling Behavior Noam Miller and Robert Gerlai 17. Assessing Social Behavior Phenotypes in Adult Zebrafish: Shoaling, Social Preference, and Mirror Biting Tests Mimi Pham, Jolia Raymond, Jonathan Hester, Evan Kyzar, Siddharth Gaikwad, Indya Bruce, Caroline Fryar, Simon Chanin, Joseph Enriquez, Sidarth Bagawandoss, Ivan Zapolsky, Jeremy Green, Adam Michael Stewart, Barrie D. Robison, and Allan V. Kalueff 18. Measuring Effects of Psychostimulants on Egocentric Spatial Learning and Memory in Adult Zebrafish Rabia Nasir, Tenzin Choezom, Jennifer Cunningham, Benjamin Bajaj, Cristina Rubi, Jonathan Butler, Kathryn Arroyo, and Joseph A. Schroeder 19. Cued Fear Conditioning in Zebrafish (Danio rerio) Masakazu Agetsuma, Tazu Aoki, Ryo Aoki, and Hitoshi Okamoto 20. Assessing Learning and Memory Through the Active Avoidance Paradigm Xiaojuan Xu and Stefan Goetz 21. Assessing Habituation Phenotypes in Adult Zebrafish: Intra- and Inter-Trial Habituation in the Novel Tank Test Jolia Raymond, Simon Chanin, Adam Michael Stewart, Evan Kyzar, Siddharth Gaikwad, Andrew Roth, Indya Bruce, Caroline Fryar, Danielle Varga, Joseph Enriquez, Sidarth Bagawandoss, Mimi Pham, Ivan Zapolsky, Jeremy Green, Daniel Desmond, Jonathan Hester, and Allan V. Kalueff 22. Assessing Startle Responses and Their Habituation in Adult Zebrafish Simon Chanin, Caroline Fryar, Danielle Varga, Jolia Raymond, Evan Kyzar, Joseph Enriquez, Sidarth Bagawandoss, Siddharth Gaikwad, Andrew Roth, Mimi Pham, Ivan Zapolsky, Indya Bruce, Jonathan Hester, Jeremy Green, Daniel Desmond, Adam Michael Stewart, and Allan V. Kalueff 23. QTL Mapping Using Behavioral Traits in the Adult Zebrafish Dominic Wright 24. Assessing Epilepsy-Related Behavioral Phenotypes in Adult Zebrafish Daniel Desmond, Evan Kyzar, Siddharth Gaikwad, Jeremy Green, Russell Riehl, Andrew Roth, Adam Michael Stewart, and Allan V. Kalueff 25. Rapid Method for Acute Intracerebroventricular Injection in Adult Zebrafish Augusto Barbosa, Jr., Caio Maximino, Aparecida de Souza Fim Pereira, Carla Cristina Bejo Wolkers, Fabiana Luca Alves, Liliam Midori Ide, Anderson Manoel Herculano, and Anette Hoffmann 26. Western Blotting Assay for Egr-1 Immediate Early Gene in Brain Tissue of Zebrafish Applied to Neuroethological Study Augusto Barbosa, Jr., Anette Hoffmann, Fabiana Luca Alves, Carla Cristina Bejo Wolkers, Fernando Massaru Hoshiko, Liliam Midori Ide, Lilian Eslaine Costa Mendes Silva, and Paula Santos 27. Utilizing the Zebrafish Neurophenome Project (ZNP) Database for Analyses of Complex Neurophenotypes in Zebrafish Models Ivan Zapolsky, Evan Kyzar, Jeremy Green, Siddharth Gaikwad, Mimi Pham, Simon Chanin, Caroline Fryar, Jonathan Hester, Sidarth Bagawandoss, Jolia Raymond, Joseph Enriquez, Adam Michael Stewart, and Allan V. Kalueff Dominic Wright 24. Assessing Epilepsy-Related Behavioral Phenotypes in Adult Zebrafish Daniel Desmond, Evan Kyzar, Siddharth Gaikwad, Jeremy Green, Russell Riehl, Andrew Roth, Adam Michael Stewart, and Allan V. Kalueff 25. Rapid Method for Acute Intracerebroventricular Injection in Adult Zebrafish Augusto Barbosa, Jr., Caio Maximino, Aparecida de Souza Fim Pereira, Carla Cristina Bejo Wolkers, Fabiana Luca Alves, Liliam Midori Ide, Anderson Manoel Herculano, and Anette Hoffmann 26. Western Blotting Assay for Egr-1 Immediate Early Gene in Brain Tissue of Zebrafish Applied to Neuroethological Study Augusto Barbosa, Jr., Anette Hoffmann, Fabiana Luca Alves, Carla Cristina Bejo Wolkers, Fernando Massaru Hoshiko, Liliam Midori Ide, Lilian Eslaine Costa Mendes Silva, and Paula Santos 27. Utilizing the Zebrafish Neurophenome Project (ZNP) Database for Analyses of Complex Neurophenotypes in Zebrafish Models Ivan Zapolsky, Evan Kyzar, Jeremy Green, Siddharth Gaikwad, Mimi Pham, Simon Chanin, Caroline Fryar, Jonathan Hester, Sidarth Bagawandoss, Jolia Raymond, Joseph Enriquez, Adam Michael Stewart, and Allan V. Kalueff Daniel Desmond, Evan Kyzar, Siddharth Gaikwad, Jeremy Green, Russell Riehl, Andrew Roth, Adam Michael Stewart, and Allan V. Kalueff 25. Rapid Method for Acute Intracerebroventricular Injection in Adult Zebrafish Augusto Barbosa, Jr., Caio Maximino, Aparecida de Souza Fim Pereira, Carla Cristina Bejo Wolkers, Fabiana Luca Alves, Liliam Midori Ide, Anderson Manoel Herculano, and Anette Hoffmann 26. Western Blotting Assay for Egr-1 Immediate Early Gene in Brain Tissue of Zebrafish Applied to Neuroethological Study Augusto Barbosa, Jr., Anette Hoffmann, Fabiana Luca Alves, Carla Cristina Bejo Wolkers, Fernando Massaru Hoshiko, Liliam Midori Ide, Lilian Eslaine Costa Mendes Silva, and Paula Santos 27. Utilizing the Zebrafish Neurophenome Project (ZNP) Database for Analyses of Complex Neurophenotypes in Zebrafish Models Ivan Zapolsky, Evan Kyzar, Jeremy Green, Siddharth Gaikwad, Mimi Pham, Simon Chanin, Caroline Fryar, Jonathan Hester, Sidarth Bagawandoss, Jolia Raymond, Joseph Enriquez, Adam Michael Stewart, and Allan V. Kalueff Augusto Barbosa, Jr., Caio Maximino, Aparecida de Souza Fim Pereira, Carla Cristina Bejo Wolkers, Fabiana Luca Alves, Liliam Midori Ide, Anderson Manoel Herculano, and Anette Hoffmann 26. Western Blotting Assay for Egr-1 Immediate Early Gene in Brain Tissue of Zebrafish Applied to Neuroethological Study Augusto Barbosa, Jr., Anette Hoffmann, Fabiana Luca Alves, Carla Cristina Bejo Wolkers, Fernando Massaru Hoshiko, Liliam Midori Ide, Lilian Eslaine Costa Mendes Silva, and Paula Santos 27. Utilizing the Zebrafish Neurophenome Project (ZNP) Database for Analyses of Complex Neurophenotypes in Zebrafish Models Ivan Zapolsky, Evan Kyzar, Jeremy Green, Siddharth Gaikwad, Mimi Pham, Simon Chanin, Caroline Fryar, Jonathan Hester, Sidarth Bagawandoss, Jolia Raymond, Joseph Enriquez, Adam Michael Stewart, and Allan V. Kalueff Augusto Barbosa, Jr., Anette Hoffmann, Fabiana Luca Alves, Carla Cristina Bejo Wolkers, Fernando Massaru Hoshiko, Liliam Midori Ide, Lilian Eslaine Costa Mendes Silva, and Paula Santos 27. Utilizing the Zebrafish Neurophenome Project (ZNP) Database for Analyses of Complex Neurophenotypes in Zebrafish Models Ivan Zapolsky, Evan Kyzar, Jeremy Green, Siddharth Gaikwad, Mimi Pham, Simon Chanin, Caroline Fryar, Jonathan Hester, Sidarth Bagawandoss, Jolia Raymond, Joseph Enriquez, Adam Michael Stewart, and Allan V. Kalueff Ivan Zapolsky, Evan Kyzar, Jeremy Green, Siddharth Gaikwad, Mimi Pham, Simon Chanin, Caroline Fryar, Jonathan Hester, Sidarth Bagawandoss, Jolia Raymond, Joseph Enriquez, Adam Michael Stewart, and Allan V. Kalueff